论文部分内容阅读
A novel glucose biosensor was constructed by immobilizing the glucose oxidase(GOD) on a hydroxyapatite(HAp)/Nafion composite film modified glassy carbon electrode(GCE) and applied to the highly selective and sensitive determination of glucose.With the cooperation of HAp and Nafion,the composite film played an important role in enhancing the stability and sensitivity of the biosensor.The results demonstrate that the GOD adsorbed onto the HAp/Nafion composite film exhibits a pair of welldefined nearly reversible redox peaks and fine catalysis to the oxidation of glucose companied with the consumption of dissolved oxygen.On the basis of the decrease of the reduction current of dissolved oxygen at the applied potential of -0.80 V(vs.SCE) upon the addition of glucose,the concentration of glucose could be detected sensitively and selectively.The decreased reduction current was linear with the concentration of glucose in the range of 0.12―2.16 mM.The detection limit and sensitivity were 0.02 mM(S/N=3) and 6.75 mA·M-1,respectively.All the results demonstrate that HAp/Nafion composite film provides a novel and efficient platform for the immobilization of enzymes and realizes the direct electrochemistry.The composite materials should have potential applications in the fabrication of third-generation biosensors.
A novel glucose biosensor was constructed by immobilizing the glucose oxidase (GOD) on a hydroxyapatite (HAp) / Nafion composite film modified glassy carbon electrode (GCE) and applied to the highly selective and sensitive determination of glucose. The cooperation of HAp and Nafion , the composite film played an important role in enhancing the stability and sensitivity of the biosensor. The results demonstrate that the GOD adsorbed onto the HAp / Nafion composite film exhibits a pair of welldefined nearly reversible redox peaks and fine catalysis to the oxidation of glucose companied with the consumption of dissolved oxygen. On the basis of the decrease of the reduction current of dissolved oxygen at the applied potential of -0.80 V (vs.SCE) upon the addition of glucose, the concentration of glucose could be detected sensitively and selectively. The decreased reduction current was linear with the concentration of glucose in the range of 0.12-2.16 mM. The detection limit and sensitivity were 0.0 2 mM (S / N = 3) and 6.75 mA · M-1, respectively.All the results demonstrate that HAp / Nafion composite film provides a novel and efficient platform for the immobilization of enzymes and realizes the direct electrochemistry. have potential applications in the fabrication of third-generation biosensors.