氟虫腈高效液相色谱分析方法优化

• 作 者: 王玉玲 史常春 李自华 夏禹 吴伍涛
• 机 构: 荆州市产品质量监督检验所,
• 出 处: 农药
• 发表日期: 2014年08期
• 关键词: 氟虫腈 检出限 固定相 高效液相色谱 色谱柱 三唑磷乳油 杀虫剂 阿维菌素 流动相 高效液相色谱仪

[目的]对农药中添加的氟虫腈进行高效液相色谱分析条件的优化。[方法]用二级管阵列检测器,C18柱为固定相,以乙腈-甲醇-0.05%磷酸为流动相(体积比45∶20∶35)。[结果]该方法分离效果较好,检出限0.5 mg/L,平均回收率为98.6%~102.8%。[结论]该方法检出限低,回收率高,可满足各种类型农药中氟虫腈的检测。

[Objective] The research aimed to optimize the conditions of high performance liquid chromatographic analysis of fipronil added to pesticides. [Method] With diode array detector, C18 column was used as stationary phase and acetonitrile - methanol - 0.05% phosphoric acid was used as mobile phase (volume ratio 45:20:35). [Result] The method had good separation efficiency with the detection limit of 0.5 mg / L and the average recovery of 98.6% -102.8%. [Conclusion] The method has low detection limit and high recovery rate, which can meet the detection of fipronil in various types of pesticides.