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目的:研究MK801对大鼠脑缺血再灌注后海马CA1区c-fos基因表达及神经元凋亡的影响。方法:将大鼠随机分为正常对照组、脑缺血再灌注模型组和脑缺血用药后再灌注组,用药组在再灌注前经腹腔注射MK801(0.5mg/kg);分别于再灌注后2、6、24和48 h,采用免疫组化法和Western印迹观察海马CA1区c-fos基因的表达情况。结果:c-fos基因的表达在脑缺血再灌注后2 h即开始增强,至再灌注后6 h达到高峰,24 h表达降低,至48 h又至高峰,但较再灌注6 h后较低。用药组海马CA1区c-fos基因的表达均较模型组的相应时间段降低,具有显著性差异(P<0.01)。结论:海马CA1区c-fos基因在脑缺血再灌注后表达升高,MK801可降低脑缺血再灌注后c-fos基因的表达,对缺血再灌注后的脑组织具有保护作用。
AIM: To investigate the effect of MK801 on c-fos gene expression and neuronal apoptosis in hippocampal CA1 subfields after cerebral ischemia-reperfusion in rats. Methods: The rats were randomly divided into normal control group, cerebral ischemia-reperfusion model group and reperfusion group after cerebral ischemia administration. The treatment group was intraperitoneally injected MK801 (0.5mg / kg) before reperfusion, The expression of c-fos gene in hippocampal CA1 region was observed by immunohistochemistry and Western blotting at 2, 6, 24 and 48 h after the operation. Results: The expression of c-fos gene began to increase 2 h after cerebral ischemia-reperfusion, peaked at 6 h after reperfusion, decreased at 24 h, peaked at 48 h, but peaked at 48 h low. The expression of c-fos gene in hippocampal CA1 region of the treatment group was significantly lower than that of the model group (P <0.01). CONCLUSION: The expression of c-fos gene in hippocampal CA1 region is increased after cerebral ischemia-reperfusion. MK801 can reduce the expression of c-fos gene after cerebral ischemia and reperfusion, and has a protective effect on brain tissue after ischemia-reperfusion.