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采用检测血管紧张素转换酶(ACE)活性的高效液相色谱法,对自制猪肺ACE酶催化反应条件进行优化,结果表明ACE与底物HGG的最佳反应条件是:底物HGG和ACE的最适反应浓度分别为5mmol/L和12.5mg/mL,反应时间30min。以水解度(DH)为指标,测定时间因素对不同蛋白酶酶解牡蛎蛋白的影响,结果表明:4h时水解基本完全,中性蛋白酶水解度最高,达到89.88%;木瓜蛋白酶水解度最低,为34.51%。探讨了牡蛎蛋白的水解度与ACE抑制活性的关系,结果表明:水解15min后,酶解产物ACE抑制率达较高水平,随时间延长而缓慢增加或减少;其中胃蛋白酶水解牡蛎蛋白酶解物对ACE的抑制率最高,1200U/g底物的添加量、水解4h的条件下,其抑制率可达96.99%,且酶解物可在12h内保持稳定而不被水解。
The conditions of ACE enzyme catalyzed reaction in porcine lung were optimized by high performance liquid chromatography (HPLC). The results showed that the optimum reaction conditions of ACE and substrate HGG were as follows: substrate HGG and ACE The optimum reaction concentrations were 5mmol / L and 12.5mg / mL respectively, and the reaction time was 30min. The degree of hydrolysis (DH) was used as an index to determine the effect of time on the proteolysis of oysters by different proteases. The results showed that the hydrolysis was basically complete at 4h, the neutral protease reached the highest degree of 89.88%, and the lowest degree of papain hydrolysis was 34.51 %. The relationship between the degree of hydrolysis of oyster protein and the ACE inhibitory activity was discussed. The results showed that after 15 min of hydrolysis, the ACE inhibitory rate of the hydrolyzate reached a high level and slowly increased or decreased with the prolongation of time. The effect of pepsin hydrolysis on the ACE inhibitory activity The inhibition rate of ACE was the highest. Under the condition of 1200U / g substrate addition and hydrolysis for 4h, the inhibition rate reached 96.99%, and the hydrolyzate could be stable within 12h without being hydrolyzed.