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目的:测定5个不同产地的茯苓与猪苓中无机元素的含量,探讨无机元素含量的地域差异性及药材中无机元素间的相关性,并探讨利尿作用与元素含量的相关性。方法:采用微波消解-电感耦合等离子体质谱法(ICP-MS)对茯苓与猪苓中的无机元素进行全定量分析。应用MPP(12.6)软件对数据进行主成分分析(PCA)及聚类分析,SPSS 17.0软件对元素含量的相关性进行分析。结果:含量测定结果表明茯苓与猪苓中各无机元素的含量随原子序数增大呈现相似的规律性分布;主产地的茯苓与猪苓中无机元素含量高于其他产地。主成分分析和聚类分析表明,2种药材间无机元素的含量差异大,云南、广西与贵州茯苓间无机元素的含量相近,湖南与安徽茯苓间无机元素含量相近;山西、陕西与安徽猪苓中无机元素的含量相近,辽宁与吉林猪苓中无机元素的含量相近。相关性结果表明茯苓和猪苓中有55对元素具有相关性(P<0.05),有121对元素具有显著相关性(P<0.01);常量元素Mg与Na,K显著相关,绝大部分的微量元素之间具有显著相关性;常量元素与微量元素之间也具有显著的相关性。结论:该方法适合茯苓、猪苓药材中多种元素含量的同时测定;药材的种属亲缘关系及药材的资源品质与地域性密切相关;药材对无机元素的吸收具有选择性,其吸收比例可能与药材的药理活性及强弱有关。
OBJECTIVE: To determine the contents of inorganic elements in Poria cocos and Polyporus umbellatus from five different habitats, and to explore the geographical differences of inorganic elements and the correlation between inorganic elements in herbs, and to explore the correlation between diuretic effects and elemental contents. Methods: The inorganic elements in Poria and Polyporus umbellatus were quantitatively analyzed by microwave digestion-inductively coupled plasma mass spectrometry (ICP-MS). The data were analyzed by principal component analysis (PCA) and cluster analysis using MPP software (12.6) and SPSS 17.0 software. RESULTS: The content of inorganic elements in Poria cocos and Polyporus umbellatus showed similar regular distribution with the increase of atomic number. The contents of inorganic elements in Poria cocos and Polyporus umbellatus were higher than those in other areas. The results of principal component analysis and cluster analysis showed that the contents of inorganic elements in the two medicinal materials were quite different. The contents of inorganic elements in Yunnan, Guangxi and Guizhou were similar, and the contents of inorganic elements in Hunan and Anhui were similar. The contents of inorganic elements in Hunan and Anhui were similar. The contents of inorganic elements in Liaoning and Jilin are similar. Correlation results showed that there were 55 pairs of elements in Poria and Polyporus (P <0.05), and 121 pairs of elements had significant correlation (P <0.01). The major element Mg was significantly related to Na and K, most of which Trace elements have a significant correlation between; constant elements and trace elements also have significant correlations. Conclusion: The method is suitable for the simultaneous determination of the content of many elements in Poria and Polyporus umbellatus. The genetic relationship of medicinal plants and the resource quality of medicinal materials are closely related to the regionality. The absorption of inorganic elements by medicinal herbs is selective, and the absorption ratio may be Pharmacological activity and strength and weakness related.