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目的了解食用煤氟污染的粮食对动情期雌鼠全血铜(Cu)、锌(Zn)、铁(Fe)、钙(Ca)、镁(Mg)元素的影响。方法选取贵阳医学院动物实验中心提供的90只断乳2周、体重60~80 g的SD雌性大鼠为研究对象,随机分为对照组、中氟组、高氟组,每组各30只。各染氟组喂饲含不同比例的燃煤型氟中毒病区煤烘烤玉米(含氟量分别为47.8 mg/kg、96 mg/kg)复制燃煤型氟中毒模型,对照组玉米饲料来自非病区(含氟量为5.2 mg/kg)。分别于染氟60、120、180天时于股动脉取血,检测Cu、Zn、Fe、Ca、Mg元素水平。结果 60天时染氟组Cu、Zn、Fe明显降低,高氟组Ca明显增高,差异有统计学意义(P均<0.05);3组Mg随染氟剂量增加而降低,差异无统计学意义(P>0.05)。120天时染氟组Cu均升高;高氟组Zn高于对照组、中氟组Zn随染氟剂量增加而升高,差异有统计学意义(P<0.05);120天时高氟组Fe降低,差异有统计学意义(P<0.05)。180天时染氟组Cu、Zn、Fe、Ca、Mg接近对照组水平,差异无统计学意义(P>0.05)。染氟组Cu在120、180天高于60天,差异有统计学意义(P<0.05),且随染氟时间的延长逐渐升高。染氟组Zn在120、180天高于60天,差异有统计学意义(P<0.05),高氟组Zn在180天低于120天,差异有统计学意义(P<0.05)。染氟组Fe在120、180天高于60天,随着中毒时间延长逐渐升高,除中氟组120、180天之间差异无统计学意义外(P>0.05),其余各组间比较差异均有统计学意义(P<0.05)。中氟组Ca在120、180天高于60天,随染毒时间延长,全血Ca含量逐渐增加,仅在60、180之间差异有统计学意义(P<0.05);高氟组60、120、180天全血Ca含量基本维持同一水平,差异无统计学意义(P>0.05)。3组Mg均随时间延长而逐渐增加,差异有统计学意义(P<0.05)。结论燃煤型氟中毒可导致动情期雌鼠全血Cu、Zn、Fe、Ca、Mg水平紊乱,不同染氟剂量在不同染氟时间对这些元素影响错综复杂。
Objective To investigate the effect of food contaminated with coal fluoride on the contents of Cu, Zn, Fe, Ca and Mg in estrus female rats. Methods Ninety SD weaned male rats weighing 60-80 g provided by Animal Experimental Center of Guiyang Medical College were randomly divided into control group, middle fluoride group and high fluoride group, with 30 rats in each group . The coal-fumigated corn containing different proportions of coal-fired fluorosis area (containing 47.8 mg / kg fluorine and 96 mg / kg respectively) Non-ward (fluorine content of 5.2 mg / kg). Blood samples were taken from the femoral artery at 60, 120 and 180 days after fluoride exposure. The levels of Cu, Zn, Fe, Ca and Mg were measured. Results After 60 days, the contents of Cu, Zn and Fe in the fluoridation group were significantly decreased, while Ca in the high fluoride group was significantly increased (all P <0.05). The Mg in the three groups decreased with the fluorosis dose increasing, with no significant difference P> 0.05). The levels of Zn in high fluoride group were higher than those in control group at 120 days, while Zn in middle fluoride group increased with the increase of fluoride dose (P <0.05) , The difference was statistically significant (P <0.05). At 180 days, the contents of Cu, Zn, Fe, Ca and Mg in the fluoride-exposed group were similar to those in the control group, with no significant difference (P> 0.05). The content of Cu in the fluoridated group was higher than that on the 120th and 180th days, the difference was statistically significant (P <0.05), and gradually increased with the extension of the fluoridation time. The Zn content in fluoride-exposed group was higher than that on the 60th day at 120,180 days (P <0.05), and Zn in high fluoride group was lower than 120 days at 180 days (P <0.05). The fluoride content in Fe group was higher than 60 days at 120,180 days, and gradually increased with the prolongation of poisoning time. There was no significant difference except 120,180 days in fluoride group (P> 0.05) The differences were statistically significant (P <0.05). The content of Ca in middle fluorine group was higher than 60 days at 120,180 days. With the prolongation of exposure time, the content of Ca in whole blood increased gradually (P <0.05) The level of Ca in whole blood of 120 and 180 days maintained the same level, with no significant difference (P> 0.05). Mg in all three groups increased gradually with time increasing, the difference was statistically significant (P <0.05). Conclusions Coal-burning fluorosis can cause Cu, Zn, Fe, Ca and Mg levels in whole blood of estrus females to be disorderly. The effects of different dosage of fluoride on these elements are complicated at different times.