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目的了解流行性感冒病毒(流感)的流行情况,探讨流感流行规律,为制订流感控制措施提供依据。方法对乌鲁木齐市监测门诊及疫情点的疑似流感样病例采集咽拭子标本,用核酸检测和接种狗肾传代细胞(MDCK)培养进行流感病毒分离及进行血凝试验,并对阳性者进行血凝抑制试验进行分型鉴定。结果 2009年10月~2010年3月监测流感样标本与咽拭子标本共1 218份,分离出流感病毒181株,分离率3.72%,分别为甲型H1N1亚型8株、H3N2亚型32株、新甲型H1N1 104株、B型37株。结论 2009年10月~2010年1月是以新甲型H1N1亚型流感病毒流行为主,2010年2月以B型流感流行为主。
Objective To understand the epidemic situation of influenza virus (influenza), explore the epidemic rule of influenza and provide the basis for formulating influenza control measures. Methods Throat swab samples were collected from suspected influenza-like cases in outpatients and outbreaks in Urumqi. The influenza virus was isolated and hemagglutinated by nucleic acid detection and vaccination of canine kidney cell line (MDCK), and the patients were positive for hemagglutination Inhibition test for type identification. Results A total of 1 218 samples of influenza-like specimens and throat swabs were collected from October 2009 to March 2010, and 181 strains of influenza viruses were isolated with a separation rate of 3.72%, including 8 strains of type A H1N1 and 3 strains of H3N2 subtype 32 Strain, the new A H1N1 104 strains, B type 37 strains. Conclusions From October 2009 to January 2010, the influenza A (H1N1) virus is the predominant influenza virus. In February 2010, it was predominantly influenza B (influenza B).