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目的观察吗啡耐受形成过程中N-甲基-D天冬氨酸受体拮抗剂氯胺酮对小鼠脊髓诱导型一氧化氮合酶(iNOS)mRNA表达的影响。方法昆明种小鼠30只,随机分为5组(n=6):对照组(A 组)皮下注射生理盐水(10 ml·kg-1)30 min后腹腔注射生理盐水(10 ml·kg-1);慢性吗啡耐受组(B组) 皮下注射吗啡10 mg·kg-1后30min腹腔注射生理盐水10 ml·kg-1;C、D、E组吗啡用药均同B组,注射吗啡后30 min分别腹腔注射5、10、20 mg·kg-1氯胺酮。上述各组每日用药2次,连续9 d。最后1次注射吗啡后1 h处死小鼠,提取腰膨大部位脊髓组织中总RNA,以β-actin为内对照,用半定量反转录聚合酶链反应技术测定iNOS mRNA的表达量。结果 A组未见iNOS mRNA表达,其他各组可见iNOS mRNA表达;与B组相比,C、D、E组iNOS mRNA表达减少(P<0．05);与C组相比,D、E组iNOS mRNA 表达减少(P<0．05)。结论氯胺酮拮抗小鼠吗啡耐受形成与其下词脊髓iNOS mRNA表达有关。
Objective To observe the effect of ketamine, an N-methyl-D aspartate receptor antagonist, on the expression of inducible nitric oxide synthase (iNOS) mRNA in the spinal cord during morphine tolerance. Methods Thirty Kunming mice were randomly divided into 5 groups (n = 6): control group (group A) injected normal saline (10 ml · kg-1) 1). The rats in chronic morphine tolerant group (group B) were injected morphine 10 ml · kg-1 intraperitoneally 30 min after morphine 10 mg · kg-1. 30 min were intraperitoneal injection of 5,10,20 mg · kg-1 ketamine. The above groups were treated twice daily for 9 days. The mice were sacrificed 1 h after the last injection of morphine, and the total RNA was extracted from spinal cord of most lumbar spines. The expression of iNOS mRNA was determined by semi-quantitative reverse transcription-polymerase chain reaction with β-actin as internal control. Results There was no iNOS mRNA expression in group A and iNOS mRNA expression in other groups. Compared with group B, iNOS mRNA expression in group C, D and E decreased (P <0.05). Compared with group C, D and E Group iNOS mRNA expression decreased (P <0.05). Conclusion Ketamine antagonizes the morphine tolerance in mice and correlates with the expression of iNOS mRNA in the spinal cord under ketamine.