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目的建立实验性自身免疫性肝炎(EAH)小鼠模型,并对其进行动态观察。方法以同种系S-100肝抗原与弗氏完全佐剂充分乳化后两次予小鼠腹腔注射,并观察其血清谷丙转氨酶和肝组织学分级的动态变化。将S-100肝抗原层析分离后研究T细胞的自身抗原特异性增殖反应。结果小鼠在首次腹腔免疫2wk后即可见到炎症细胞特别是淋巴细胞的浸润;首次注射后4wk后组织学改变达高峰,组织学病变的消退较慢;血清ALT水平的动态变化与组织学改变相似;体外可证实对S-100抗原的T细胞增殖反应。结论实验性自身免疫性肝炎是一种由自身反应性T细胞介导的肝炎模型,可用于自身免疫性肝炎的发病机制研究。
Objective To establish a mouse model of experimental autoimmune hepatitis (EAH) and to observe it dynamically. Methods The mice were injected intraperitoneally twice with the same kind of S-100 liver antigen and Freund’s complete adjuvant. The dynamic changes of alanine aminotransferase (AST) and liver histological grading were observed. S-100 liver antigen chromatographic separation of T cells after the self-specific antigen-specific proliferative response. Results The mice were infiltrated with inflammatory cells, especially lymphocytes after 2 weeks of the first intraperitoneal immunization. The histological changes peaked 4 weeks after the first injection, and their histological changes subsided more slowly. The dynamic changes of ALT and histological changes Similar; in vitro T-cell proliferative responses to S-100 antigen can be demonstrated. Conclusion Experimental autoimmune hepatitis is a self-reactive T cell-mediated hepatitis model that can be used to study the pathogenesis of autoimmune hepatitis.