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为进一步探讨川崎病（ＫＤ）的免疫发病机理，我们以正常健康儿童为对照，观察经ａｎｔｉ－ＣＤ３ｍＡｂ刺激不同培养时间的急性期ＫＤ患儿外周血淋巴细胞形态学改变和ＤＮＡ片段分析。结果显示，正常儿童外周血淋巴细胞培养的小时即可出现特征性ＤＮＡ片段改变，细胞发生胞浆浓缩，秆状或新月形核浓缩变化：ＫＤ病人需７２小时才出现相应改变。其外周血淋巴细胞培养２４，４８小时凋亡阳性细胞百分率较正常对照组低（Ｐ＜０．００１）。结果提示急性期ＫＤ病人外周血淋巴细胞凋亡产生延迟。并可能参与ＫＤ的发病。
To further explore the pathogenesis of KD, we used normal healthy children as control and observed morphological changes and DNA fragments analysis of peripheral blood lymphocytes in children with acute KD induced by anti-CD3 mAb at different culture time. The results showed that the characteristic DNA fragments could be changed in the hours of normal children’s peripheral blood lymphocytes, and the concentration of cytoplasm, stalk or crescent nucleus changes: KD patients need to change 72 hours. The percentage of apoptotic cells in peripheral blood lymphocytes cultured for 24,48 hours was lower than that of normal control group (P <0.001). The results suggest that acute peripheral KD patients have delayed peripheral blood lymphocyte apoptosis. And may be involved in the pathogenesis of KD.