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1974年5月—1975年1月,我们对红松(Pinus Koraiensis Sieb)进行了混砂室内自然温度催芽处理(每月一次),并于75年2—4月,两次将不同时期处理的种子作了离体胚的培养试验,研究其不同催芽时期红松胚的生长(发芽速度)情况,拟探讨红松胚有无后熟过程,以便给红松种子混砂催芽所需时间及条件提供依据。 试验方法及初步结果正理如下: 红松离体胚培养方法,基本采用李正理,张新英(1962年)试验过效果较好的培养
From May 1974 to January 1975, Pinus Koraiensis Sieb was germinated at a natural temperature in a sand chamber (once a month) and was treated twice in different periods from February to April in 1975 Seed in vitro culture experiments were carried out to study the different germination period of the growth of Korean pine embryo (germination rate), to investigate whether the Korean pine embryo ripening process in order to give the Pinus koreans seed germination time and conditions Provide evidence. The test method and the preliminary results are as follows: In vitro culture method of Korean pine, the basic use of Li Zhengli, Zhang Xinying (1962) to test the effect of better training