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以香菇( Lentinus edodes )菌丝为材料,以纤维素酶、β-葡萄糖醛酸苷酶的组合酶溶液降解菌丝细胞壁,获原生质体。香菇菌丝原生质体在含1.5g/L酵母膏、20g/L葡萄糖与0.6mol/L甘露醇的液体培养基中培养6—8h后,开始启动生长。培养24h,可观察到分枝状菌丝的形成。定点连续观察表明,不论是单向出芽生长还是双向出芽生长的再生方式,都是原生质体在培养中正常的生长方式。单向生长的芽管状菌丝、双向生长的菌丝以及多分枝的菌丝形态,是原生质体再生过程中不同生长阶段的表现。
Lentinus edodes hyphae were used as materials to degrade the mycelial cell wall with the combination of cellulase and β-glucuronidase to obtain protoplasts. Mushroom mycelium protoplasts cultured in liquid medium containing 1.5g / L yeast extract, 20g / L glucose and 0.6mol / L mannitol for 6-8h, began to start growth. After cultured for 24h, the formation of branched hyphae was observed. The continuous observation of fixed point shows that both the unidirectional sprouting and the regeneration of two-way sprouting are the normal growth modes of protoplasts in culture. Uniform growth of tubular shoot mycelium, two-way growth of mycelium and multi-branched mycelium morphology, protoplast regeneration process in different stages of performance.