对缺硼反应不同的油菜基因型根系吸收硼特性的研究

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以对缺硼敏感性不同的甘蓝型油菜品种为供试材料,研究其整体根系和离体根吸收硼特性的差异。结果表明,2个品种根系吸硼速率与外界硼浓度呈显著直线相关,直线斜率以敏感品种大于不敏感品种,无论整体根还是离体根都得到同样结果。在不同硼浓度时,2个品种整体根系吸硼速率有明显差异。低硼浓度时(B0.05~1.0μg/g),敏感品种吸硼速率显著低于不敏感品种;而高硼浓度时(B>1.0μg/g),则明显高于不敏感品种;采用离体根测定发现,硼进入2个品种根系自由空间过程符合Michaelis-Menten方程,但敏感品种的Km值高于不敏感品种,Vmax则小于不敏感品种。不敏感品种在低硼浓度时吸收能力强以及对硼的亲和力高可能是其硼高效的重要原因。 Taking different Brassica napus cultivars susceptible to boron deficiency as test materials, the differences of boron absorption characteristics between the whole roots and ex vivo roots were studied. The results showed that there was a significant linear correlation between the boron uptake rate and the boron concentration in the two cultivars. The slope of the line was more sensitive than the insensitive ones, and the same result was obtained both in the whole root and in vitro. At different boron concentrations, there was a significant difference in boron uptake rate between the two cultivars. At low boron concentration (B0.05 ~ 1.0μg / g), the boron uptake rate of the susceptible varieties was significantly lower than that of the non-sensitive varieties; while the high boron concentration (B> 1.0μg / g) In vitro root determination, it was found that boron entering the root free space of two cultivars accorded with Michaelis-Menten equation, but Km value of sensitive species was higher than that of non-sensitive species and Vmax was less than that of non-sensitive species. Insensitive varieties with high absorption capacity at low boron concentrations and high affinity for boron may be important reasons for their high boron efficiency.
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