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在实验室条件下,测定了昆虫病原真菌玫烟色棒束孢Isaria fumosorosea的侵染对小菜蛾Plutella xylostella3龄幼虫体内酚氧化酶(PO)和不同抗氧化酶类,包括超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢(CAT)及谷胱甘肽S-转移酶(GSTs)活力的变化。结果表明,玫烟色棒束孢侵染小菜蛾3龄幼虫后,体内PO、SOD、POD、CAT和GSTs活力均受到明显的影响。其中感病小菜蛾幼虫体内的PO活力始终高于同期未感染的小菜蛾幼虫,当接菌40h时,酚氧化酶活力达到最大37.4U/g,为对照的2.6倍,而SOD、POD、CAT和GSTs活力在感病前期明显高于对照,接菌40–48h各种酶活力均达到最大,当接菌56h酶活力开始下降,64h时酶活力均明显低于对照。可见玫烟色棒束孢的侵染严重干扰了小菜蛾幼虫体内正常的生理活动。
Under laboratory conditions, the effects of Isaria fumosorosea, an entomopathogenic fungus, on the activities of phenoloxidase (PO) and antioxidant enzymes in Plutella xylostella 3-instar larvae, including superoxide dismutase SOD, POD, CAT and GSTs were measured. The results showed that the 3rd instar larvae of Plutella xylostella were affected by PO, SOD, POD, CAT and GSTs activity obviously. The activity of PO in susceptible Plutella xylostella larvae was always higher than that of the uninfected Plutella xylostella larvae at the same period. The maximum activity of phenoloxidase reached 37.4U / g at 40h after inoculation for 40h, which was 2.6 times of the control, while the activities of SOD, POD, CAT The activity of GSTs was significantly higher than that of the control at the early stage of infection. The activities of all enzymes reached the maximum after 40-48 hours of inoculation. When 56 hours of inoculation began to decrease, the activity of GSTs was significantly lower than that of the control at 64 hours. It can be seen that the infection of Corynebacterium roseum severely interfered with the normal physiological activity of Plutella xylostella larvae.