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目的探讨白细胞介素1β(IL~(-1)β)-511C/T和+3954C/T与无症状慢性阻塞性肺疾病(无症状COPD)的相关性。方法收集2009年1月~2013年6月河北联合大学附属开滦总医院呼吸科门诊筛查的300例无症状COPD患者及320例同期健康对照人群,用酶联免疫吸附试验(ELISA)检测血清IL~(-1)β水平,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测-511C/T、+3954C/T位点的基因型。结果无症状COPD组的血清IL~(-1)β水平为(3.93±0.44)ng·m L~(-1),对照组为(2.73±0.12)ng·m L~(-1),组间差异具有统计学意义(P<0.001)。无症状COPD组IL~(-1)β-511的CC、CT和TT基因型频率分别为20.0%、63.0%和17.0%,对照组分别为22.2%、60.9%和16.9%,2组相比差异无统计学意义(P=0.336)。无症状COPD组IL~(-1)β+3954CC、CT和TT基因型频率分别为84.0%、16.0%和0%,对照组分别为86.9%、12.8%和0.3%,2组相比差异无统计学意义(P=0.795)。2组人群IL~(-1)β-511、+3954等位基因频率比较差异无统计学意义(P=0.684和P=0.387),且男性和女性人群亚组分析中2组人群差异均无统计学意义(P>0.05)。结论无症状COPD组血清IL~(-1)β水平高于对照组;IL~(-1)β中-511、+3954的基因型频率与等位基因频率与无症状COPD的发生无明显相关性。
Objective To investigate the relationship between interleukin-1β (IL-1β) -511C / T and + 3954C / T and asymptomatic chronic obstructive pulmonary disease (asymptomatic COPD). Methods A total of 300 asymptomatic COPD patients and 320 healthy controls were recruited from January 2012 to June 2013 in the Department of Respiratory Clinic of Kailuan General Hospital affiliated to Hebei Union University. Serum levels were detected by enzyme-linked immunosorbent assay (ELISA) The level of IL-1β was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results Serum level of IL-1β was (3.93 ± 0.44) ng · m L -1 in asymptomatic COPD group and (2.73 ± 0.12) ng · m L -1 in control group, The difference was statistically significant (P <0.001). The frequencies of CC, CT and TT genotypes of IL-1β-511 in asymptomatic COPD group were 20.0%, 63.0% and 17.0%, respectively, while those in control group were 22.2%, 60.9% and 16.9% The difference was not statistically significant (P = 0.336). The frequencies of IL-1β, 3954CC, CT and TT genotypes in asymptomatic COPD patients were 84.0%, 16.0% and 0%, respectively, while those in the control group were 86.9%, 12.8% and 0.3%, respectively Statistical significance (P = 0.795). There were no significant differences in the frequencies of IL-1β-511 and + 3954 alleles between the two groups (P = 0.684 and P = 0.387), and there was no significant difference between the two groups in subgroup analysis of male and female Statistical significance (P> 0.05). Conclusions The serum level of IL-1βin asymptomatic COPD group is higher than that in control group. There is no significant correlation between the genotype frequency of -511 and + 3954 in IL -1 (-1) β and allele frequency and the occurrence of asymptomatic COPD Sex.