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目的观察食用紫菜后的志愿者尿中各形态砷和尿总砷(TAs)的随时间的变化规律及其对尿TAs的影响。方法志愿者禁食海产品至少72h,收集食用紫菜后第1、3、5、8、12、24、36、48、60、72小时尿样;运用横断面调查方法,随机采集某渔村55名居民尿样,所有尿样采用冷阱捕集-氢化物发生原子分光光度法检测尿中各形态砷[一甲基砷(MMA)和二甲基砷(DMA)]和TAs含量。结果志愿者食用紫菜后,尿TAs随时间的延长而上升,在第36小时达到高峰,然后随时间的延长而下降,尿中DMA的变化同尿TAs的变化一致。食用紫菜后36h内女性尿中DMA的含量高于男性(P<0.05),TAs排泄高峰时间早于男性12h。渔村居民尿中MMA、DMA和尿TAs显著高于对照组居民,渔村居民尿中特异性地检出了三甲基砷(TMA)。结论志愿者食用海产品后,尿TAs会有显著的上升且尿TAs中以DMA的变化为主。女性对海产品中的砷化物的代谢和排泄能力高于男性。渔村居民尿中DMA和TMA为尿TAs的主要组成部分。
Objective To observe the changes of urine arsenic and urine total arsenic (TAs) in volunteers after eating laver and their effects on urinary TAs. Methods Volunteers fasting seafood for at least 72h, collected after eating seaweed 1,3,5,8,12,24,36,48,60,72 hours urine samples; using cross-sectional survey method, a random collection of 55 fishing villages Residents of urine samples, all urine samples were collected using cold trap - hydride generation atomic spectrophotometry urinary various forms of arsenic [monomethyl arsenic (MMA) and dimethyl arsenic (DMA)] and TAs content. Results After consuming seaweed, volunteers’ urinary TAs increased with time, peaked at 36 hours, and then decreased with time. The changes of urinary DMA were consistent with those of urine TAs. The content of urinary DMA was higher in women than in men (P <0.05) within 36 hours after eating seaweed, and the peak time of excretion of TAs was earlier than that in men. Urinary urine MMA, DMA and urine TAs were significantly higher in residents of the fishing village than in the control group. Trimethylarsine (TMA) was specifically detected in the urine of fishing village residents. Conclusions The urine TAs increased significantly in volunteers after consumption of seafood, and the changes in urine TAs were dominated by DMA. Women’s ability to metabolize and excrete arsenic from seafood is higher than that of men. The urinary DMA and TMA of fishing village residents are the main components of urine TAs.