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原子力显微术 (atomicforcemicroscopy,AFM)是一种新型的纳米显微技术 ,由于其拥有标本制备简单、分辨率高等优点 ,因此常用于细胞超微结构的观察。双微体 (doubleminutechromosomes ,DMs)是基因扩增的主要表现形式 ,经常出现在肿瘤细胞及耐药细胞中 ,可使肿瘤细胞获得生存优势或产生耐药性 ,因此对双微体进行研究可使人类了解肿瘤的生长特性及其抗药性的产生机理。为寻找一种研究双微体的有效方法 ,本实验利用原子力显微镜对小鼠耐氨甲喋呤细胞 3T3R5 0 0中的双微体进行观察 ,在获得双微体高分辨AFM形态图的同时 ,还对双微体的大小进行了测量 ,发现细胞中双微体大小存在差异。此外 ,就原子力显微镜在双微体研究中的一些技术细节进行了探讨。实验结果表明原子力显微术是研究双微体的一种有效手段。
Atomic force microscopy (atomic force microscopy, AFM) is a new type of nano-microscopy, which is commonly used in the observation of cellular ultrastructure because of its simple preparation and high resolution. Double-micro-organisms (double minutechromosomes, DMs) is the main form of gene amplification, often appear in tumor cells and drug-resistant cells, can make the survival advantage of tumor cells or drug resistance, therefore, Human understanding of tumor growth characteristics and mechanism of drug resistance. In order to find an effective method to study the double-micro-organisms, we observed the double-micro-organisms in the methotrexate-resistant cells 3T3R5 0 0 by atomic force microscopy. The size of the body was measured and it was found that there was a difference in the size of the dual microbeads in the cells. In addition, some technical details of AFM in dual-micro-studies are discussed. The experimental results show that atomic force microscopy is an effective means to study the double micro-body.