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目的 :探讨半枝莲总黄酮类提取物对乳腺癌MDA-MB-231细胞增殖、迁移、侵袭能力的影响。方法 :体外培养乳腺癌MDA-MB-231细胞株,提取并鉴定半枝莲总黄酮提取物,配制不同浓度提取物作用于细胞,分别以MTT比色法、细胞划痕实验、transwell实验检测其对MDA-MB-231细胞增殖、迁移、侵袭能力的影响,用实时定量PCR检测甲状旁腺相关蛋白(PTHr P)m RNA的表达变化。结果:与对照组相比,半枝莲总黄酮类提取物40~200μg/m L共5组作用于MDA-MB-231细胞24 h时,细胞增殖抑制率分别为(11.7±5.7)%、(25.1±4.1)%、(40.1±4.0)%、(44.7±4.0)%、(45.1±2.9)%;细胞迁移抑制率分别为(8.9±1.8)%、(20.4±2.7)%、(21.8±2.4)%、(29.3±4.0)%、(51.7±6.3)%;细胞侵袭抑制率分别为(31.4±0.3)%、(36.5±0.7)%、(57.8±0.8)%、(59.8±1.1)%、(80.7±1.4)%;PTHr P m RNA相对表达水平分别为81.8%、61.8%、46.5%、35.1%、22.2%。结论:半枝莲总黄酮类提取物可能通过下调PTHr P m RNA的表达,抑制乳腺癌MDA-MB-231细胞的增殖、迁移及侵袭能力。
Objective: To investigate the effect of Scutellaria barbata extract on the proliferation, migration and invasion of breast cancer MDA-MB-231 cells. Methods: Breast cancer cell line MDA-MB-231 was cultured in vitro. Total flavonoids of Scutellaria barbata extract was extracted and identified. Different concentrations of extract were prepared and applied to cells. MTT colorimetric assay, cell scratch assay and transwell assay On the proliferation, migration and invasion of MDA-MB-231 cells. The expression of parathyroid hormone-related protein (PTHr P) m RNA was detected by real-time quantitative PCR. Results: Compared with the control group, the inhibitory rates of Scutellaria barbata total flavonoids extract of 40 ~ 200 μg / mL in 5 groups on MDA-MB-231 cells for 24 h were (11.7 ± 5.7)%, (25.1 ± 4.1)%, (40.1 ± 4.0)%, (44.7 ± 4.0)% and (45.1 ± 2.9)% respectively. The cell migration inhibitory rates were (8.9 ± 1.8)% The cell invasion inhibition rates were (31.4 ± 0.3)%, (36.5 ± 0.7)%, (57.8 ± 0.8)%, (59.8 ± 1.1)%, (29.3 ± 4.0)% and ) And (80.7 ± 1.4)%, respectively. The relative expression levels of PTHr P m RNA were 81.8%, 61.8%, 46.5%, 35.1% and 22.2%, respectively. Conclusion: Scutellaria barbata extract may inhibit the proliferation, migration and invasion of breast cancer MDA-MB-231 cells by down-regulating the expression of PTHr P m RNA.