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将 2 1对铲鲟 (ScaphirhynchusplatorynchusRafinesque)的微卫星引物在中华鲟 (AcipensersinensisGray)基因组DNA上进行PCR扩增 ,1 4对 (约占 67% )引物得到了扩增产物 ,其中有 1 0对 (约占 48% )表现为多态性 ,但只有 2对 ( 9 5 % )引物Spl 1 0 0和Spl 1 68的多态性较高 ,且带型清晰 ,可直接作为分子标记应用于相关的研究。此外 ,对具有特异性扩增 ,但有Stutterband现象的 4对引物的部分可分离PCR产物进行了回收和测序 ,并对其中的 3对引物的序列进行了重新设计 ,最后得到两对可应用于中华鲟的引物As 1 0 0和Spl 1 70b。研究结果表明 ,对相近种的微卫星引物进行优化设计来获得一个物种的微卫星引物是一条简捷有效的途径。
Microsatellite primers of 21 pairs of Scaphirhynchusplatorynchus Rafinesque were amplified by PCR from genomic DNA of Acipensersinensis genome. 14 pairs of primers (about 67%) were used to amplify the amplified products, of which 10 Accounting for 48%) showed polymorphism, but only two pairs (95%) of primers Spl10o and Spl188 had high polymorphism and clear banding pattern, which could be directly used as molecular markers in related research . In addition, the partially isolated PCR products of 4 pairs of primers with specific amplification, but with Stutterband phenomenon were recovered and sequenced and the sequence of 3 pairs of primers therein was redesigned. Finally, two pairs of PCR products that can be applied to Chinese sturgeon primers As 1 0 0 and Spl 1 70b. The results show that it is a simple and effective way to optimize the microsatellite primers of similar species to obtain microsatellite primers of a species.