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为分析乙型肝炎病毒（ＨＢＶ）家庭内聚集感染病例的ＨＢＶ变异特点，我们对１０个家庭２５例ＨＢＶ感染者进行了分子病毒学研究，均用单链构型多态性分析（ＳＳＣＰ）ＨＢＶ外膜基因“ａ”决定簇片段的变异，ＰＣＲ－限制性片断长度多态性分析，分析ＨＢＶ前Ｃ区终码变异。结果与用聚合酶链反应（ＰＣＲ）产物直接序列分析测定的４个家庭有、无ＨＢＶ变异株感染的外膜基因序列和前Ｃ基因序列比较，从分子水平鉴定ＨＢＶ家庭内传播，同时建立了ＳＳＣＰ检测ＨＢＶ免疫逃避变异株的方法。
To analyze the variation of HBV in hepatitis B virus (HBV) co-infected cases, we conducted a molecular virological study on 25 HBV-infected individuals from 10 families. All of them were detected by single-strand conformation polymorphism analysis (SSCP) Variants of the “a” determinant of the envelope gene were analyzed by PCR-restriction fragment length polymorphism (PCR-RFLP). Results Compared with the sequence of the exonuclease and the pre-C gene in four families tested by polymerase chain reaction (PCR), HBV DNA was identified at the molecular level and HBV The method of SSCP for detecting HBV immune escape mutants.