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目的探讨葡萄酒以及同样含有酚类的葡萄汁是否对急性酒精性肝损伤具有保护作用。方法小鼠随机分为4组:对照组、酒精组、葡萄酒组和葡萄汁组,后三组每天灌胃0.4 ml30%的食用酒精,连续7 d,建立急性酒精性肝损伤模型。同时,葡萄酒组灌胃0.42 ml长城干红葡萄酒(12%vol),葡萄汁组灌胃葡萄汁(原果汁含量100%)0.42 ml,对照组与酒精组灌入0.42 ml的蒸馏水。称量实验前后小鼠体重,检测实验后血清谷丙转氨酶(ALT)活性和丙二醛(MDA)含量,并对肝脏做病理检查。结果对照组和葡萄汁组实验前后体重增加有统计学意义(P<0.01),酒精组体重变化无统计学意义,葡萄酒组体重下降有统计学意义(P<0.05);葡萄酒组与对照组、葡萄汁组相比,ALT活力增加有统计学意义(P<0.05),而葡萄汁组与对照组、酒精组的差异均无统计学意义;各组间的MDA差异均无统计学意义;病理切片结果显示,葡萄汁与对照组肝小叶结构清晰,着色良好,细胞索排列整齐,而酒精和葡萄酒组肝小叶界限不清,排列紊乱,在中央静脉周围出现大量脂肪空泡。结论葡萄酒对急性酒精性肝损伤小鼠有损害作用,葡萄汁具有保护作用。
Objective To investigate whether wine and grape juice, also containing phenols, have a protective effect on acute alcoholic liver injury. Methods The mice were randomly divided into 4 groups: control group, alcohol group, wine group and grape juice group. The latter three groups were fed with 0.4 ml of 30% edible alcohol daily for 7 days to establish acute alcoholic liver injury model. At the same time, the wines of 0.42 ml (12% vol) were instilled into the wine group, and 0.42 ml of grape juice (original juice content of 100%) was infused into the grape juice group. 0.42 ml of distilled water was poured into the control group and the alcohol group. The body weight of the mice before and after the experiment was weighed, the activity of alanine aminotransferase (ALT) and the content of malondialdehyde (MDA) in the serum were measured, and the pathological examination of the liver was done. Results The body weight of the control group and the grape juice group increased significantly before and after the experiment (P <0.01), while there was no significant difference in body weight between the alcohol group and the grape wine group (P <0.05) Compared with grape juice group, ALT activity increased significantly (P <0.05), but no significant difference between grape juice group and control group and alcohol group. There was no significant difference in MDA between groups The results of slicing showed that the hepatic lobules of the grape juice and the control group had a clear structure with good coloration and cell lines arranged neatly. However, the hepatic lobules in the alcohol and wine groups had unclear boundaries and disordered arrangement with a large number of fat vacuoles around the central veins. Conclusion Wine has a damaging effect on mice with acute alcoholic liver injury and grape juice has a protective effect.