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作者在一次呼吸道合胞病毒(RSV)暴发流行期间,对一所医院内婴儿室的82名新生儿作了病毒学和临床表现的研究。他们每2—4天用HEP-2细胞株接种一次新生儿及保育员的鼻腔洗液,经33℃旋转培养,逐日观察RSV典型细胞病变,分离的RSV用补体结合试验予以鉴定。同时结合新生儿病历和保育员的呼吸道病调查进行分析。实验结果;在66名住院6天或6天以上的新生儿中,有23名感染了RSV,占35%,临床表现
During an epidemic of respiratory syncytial virus (RSV) outbreaks, the authors conducted a virological and clinical study of 82 neonates in a hospital infantile room. They inoculated the nasal washings of neonates and nurseries with HEP-2 cell lines every 2-4 days. The typical RSV cytopathic changes were observed daily by rotary culture at 33 ° C. The isolated RSVs were identified by complement fixation assay. At the same time, the neonatal medical record and the nurse’s respiratory disease investigation were analyzed. Results: Among 66 newborns who were hospitalized for 6 days or more, 23 were infected with RSV (35%), and their clinical manifestations