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目的:检测胃癌组织中钙指蛋白139(znic finger protein 139,ZNF139)基因的表达和RUNX3基因的甲基化状态,探讨两者之间的相关关系及其生物学意义。方法:对55例胃癌组织及相应的癌旁组织采用逆转录-聚合酶链反应(RT-PCR)检测ZNF139mRNA的表达情况,甲基化特异性PCR技术检测RUNX3基因的甲基化状态,分析两者之间的相关性。结果:ZNF139在胃癌组织中表达(0.659 2±0.229 1)明显高于癌旁组织(0.354 0±0.083 6),t=9.281,P<0.001;胃癌组织ZNF139表达与肿瘤大小有关,t=2.453,P=0.017;与分化程度有关,t=5.162,P<0.001;与浸润深度有关,t=4.145,P<0.001;与淋巴结转移有关,t=4.279,P<0.001。胃癌组织Runx3甲基化率为54.55%,明显高于癌旁组织(7.27%),χ2=28.778,P<0.001;胃癌组织Runx3基因的甲基化水平与肿瘤分化程度有关,χ2=3.911,P=0.048;与浸润深度有关,χ2=7.333,P=0.007;与淋巴结转移有关,χ2=10.530,P=0.001。发生Runx3基因甲基化的胃癌组织ZNF139表达强度(0.803 0±0.195 2)明显高于未发生Runx3甲基化的为胃癌组织(0.486 6±0.123 1),t=7.017,P<0.001,胃癌组织中Runx3基因的甲基化状态和ZNF139的表达强度呈明显正相关性,t=1.000,Kappa=0.707。结论:胃癌组织中存在ZNF139表达上调及Runx3基因甲基化率增高现象,且两者存在高度相关的一致性,提示ZNF139表达上调及Runx3基因甲基化可能通过协同作用参与了胃癌的进展及转移。
OBJECTIVE: To detect the expression of znic finger protein 139 (ZNF139) gene and the methylation status of RUNX3 gene in gastric cancer and to explore the relationship between them and their biological significance. Methods: The expression of ZNF139 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) in 55 cases of gastric cancer tissues and their corresponding paracancerous tissues. The methylation status of RUNX3 gene was detected by methylation-specific PCR. The correlation between. Results: The expression of ZNF139 in gastric cancer was significantly higher than that in adjacent tissues (0.354 ± 0.083 6, t = 9.281, P <0.001). The expression of ZNF139 in gastric cancer was related to tumor size (t = 2.453, T = 4.145, P <0.001). It was correlated with lymph node metastasis, t = 4.279, P <0.001. The methylation rate of Runx3 in gastric cancer tissues was 54.55%, significantly higher than that in adjacent tissues (7.27%), χ2 = 28.778, P <0.001. The methylation level of Runx3 in gastric cancer tissues was related to the degree of tumor differentiation (χ2 = 3.911, P = 0.048; and depth of invasion, χ2 = 7.333, P = 0.007; and lymph node metastasis, χ2 = 10.530, P = 0.001. The expression of ZNF139 (0.803 0 ± 0.195 2) in gastric cancer tissues with Runx3 gene methylation was significantly higher than that in gastric cancer tissues without Runx3 methylation (0.486 ± 0.123 1, t = 7.017, P <0.001) The methylation status of Runx3 gene was positively correlated with the expression of ZNF139, t = 1.000, Kappa = 0.707. Conclusion: The up-regulation of ZNF139 and the increase of methylation of Runx3 in gastric cancer tissues are highly consistent, suggesting that the up-regulation of ZNF139 and the methylation of Runx3 might be involved in the progression and metastasis of gastric cancer .