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【目的】烟粉虱Bemisia tabaci是一个快速进化的复合种。由于近年来烟粉虱危害不断增加,其生物型问题也越来越受到关注。在我国,不仅存在危害严重的B型烟粉虱,同时也已发现多种非B型烟粉虱。通过鉴定我国部分地区目前发生的烟粉虱生物型种类,分析它们与世界各地不同生物型之间的关系,可为我国烟粉虱的生物型检测和综合防治提供依据。【方法】利用16SrDNA基因作为分子标记,鉴定了2005年与2006年在我国6个省份(自治区)采集的22个非B型烟粉虱样本的生物型,并探讨了各生物型之间的系统进化关系。【结果】所研究的22个非B型烟粉虱归属于Q型、Nauru型和An型,3种生物型之间的遗传距离在10%以上,但是Q生物型与B生物型之间的亲缘关系最近,遗传距离在2.8%～4.0%范围内;进化分歧数据还表明,不同生物型之间的遗传距离明显大于同一生物型内遗传距离,其中Q型内部差异最小,在0.9%以内;同时结果表明目前在我国多个生物型共同存在是一个普遍的现象,其中Nauru型的分布较广泛;在云南地区检测到的非B型烟粉虱生物型类型最多。【结论】烟粉虱生物型遗传分化复杂,利用16S rDNA基因能有效鉴定烟粉虱的生物型。
【Objective】 Bemisia tabaci is a rapidly evolving complex. Due to the increasing harm of whitefly in recent years, the biotype problem is also getting more and more attention. In our country, not only the B-type B. tabaci, but also many non-B-type B. tabaci have been found. Identification of the current biotypes of B. tabaci in some parts of China and analysis of their relationships with different biotypes around the world may provide the basis for the biotype detection and comprehensive control of B. tabaci. 【Method】 Using 16S rDNA gene as a molecular marker, the biotypes of 22 non-B biotype Bemisia tabaci samples collected in 6 provinces (autonomous regions) of China in 2005 and 2006 were identified and the relationships among the biotypes Evolutionary relations. 【Result】 Twenty-two non-B biotypes of B. tabaci were classified as Q type, Nauru type and An type. The genetic distance between the three biotypes was over 10%. However, the biotypes between Q biotype and B biotype Genetic distance ranged from 2.8% to 4.0%. The data of evolution divergence also showed that the genetic distance between different biotypes was significantly greater than the genetic distance within the same biotype, with the smallest internal difference within Q type being within 0.9% At the same time, the results showed that it is a common phenomenon that multiple biotypes coexist in our country at present, of which Nauru type is more widely distributed and most of the non-B biotypes are detected in Yunnan. 【Conclusion】 The biotypes of B. tabaci were complicated by genetic differentiation. The 16S rDNA gene was used to identify the biotypes of B. tabaci.