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目的:研究罗格列酮对3T3-L1细胞内脏脂肪素表达的影响及其机制。方法:体外培养并诱导分化3T3-L1细胞,加入葡萄糖激酶制作氧化应激模型,并用不同浓度和不同作用时间罗格列酮干预,观察脂肪因子表达的变化。结果:内脏脂肪素的表达随着葡萄糖激酶氧化应激的浓度增高而递减,具有剂量依赖效应(P<0.05);内脏脂肪素的表达随着罗格列酮干预浓度增加而增加(P<0.05),随着罗格列酮作用时间的延长,内脏脂肪素的表达经历了先下降后上升的过程,且罗格列酮对于内脏脂肪素表达的影响与氧化应激状态的改变平行。结论:罗格列酮可以通过抗氧化应激作用调节内脏脂肪素的表达,可能在罗格列酮改善肥胖相关的2型糖尿病胰岛素抵抗中起到重要作用。
Objective: To investigate the effect of rosiglitazone on visfatin expression in 3T3-L1 cells and its mechanism. Methods: 3T3-L1 cells were cultured and induced in vitro. Glucose kinase was added to induce oxidative stress. The changes of adipokines expression were observed with different concentration and time of rosiglitazone intervention. Results: The expression of visfatin decreased with the increase of glucose oxidase stress (P <0.05). The visfatin increased with the increase of rosiglitazone (P <0.05) ). With the prolongation of rosiglitazone treatment, the expression of visfatin began to decline and then rose. The effect of rosiglitazone on visfatin expression was in parallel with that of oxidative stress. CONCLUSION: Rosiglitazone regulates visfatin expression through anti-oxidative stress and may play an important role in the improvement of obesity-associated insulin resistance of type 2 diabetes with rosiglitazone.