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建立了快速测定婴幼儿配方奶粉中维生素B2的高效液相色谱测定法。方法采用ZorbaxXDB-C18(5μm,4.6mm×250mmi.d)反相色谱柱,以0.05mol/L乙酸钠水溶液:甲醇(60:40,V/V,调pH值4.5)为流动相,流速为1.0mL/min,进样量10μL。方法定量限0.5mg/kg,加标回收率86.7%～91.9%,线性范围0.00～1.00μg/mL,相对标准偏差为2.12%。该法具有样品预处理简单,灵敏度高,分析时间短等优点,适用于婴幼儿配方奶粉中维生素B2的快速测定。
The rapid determination of vitamin B2 in infant formula milk by high performance liquid chromatography. Methods The mobile phase was ZorbaxXDB-C18 (5μm, 4.6mm × 250mmi.d) reversed-phase column with 0.05mol / L aqueous sodium acetate: methanol (60:40, V / V, pH = 4.5) 1.0mL / min, injection volume 10μL. The limit of quantification was 0.5 mg / kg. The recoveries were in the range of 86.7% -91.9% with a linear range of 0.00-1.00 μg / mL with a relative standard deviation of 2.12%. The method has the advantages of simple sample pretreatment, high sensitivity and short analysis time, and is suitable for the rapid determination of vitamin B2 in infant formula milk powder.