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目的:了解P物质及其受体神经激肽1(NK-1)在结肠癌中的表达及定位,探讨其在结肠癌发病及临床诊断的意义。方法:应用免疫组化方法检测正常结肠、结肠癌组织及癌旁组织中SP及NK-1的表达。采用jmtjfx10[1].31统计学软件处理数据,所得数据进行Q检验。结果:①在结肠癌组织中P物质阳性着色于胞浆,呈巢状或弥漫分布。②NK-1受体主要着色于细胞浆内,呈巢状或弥漫分布;少数为于细胞膜。③p物质与其受体NK-1在正常结肠粘膜组织及癌旁组织也有表达,位于细胞浆内,形态多呈圆形、椭圆形或不规则形。④二者在绝大多数结肠组织中呈强阳性表达,显著高于正常结肠粘膜组织和癌旁组织(p<0.01).结论:p物质与其受体NK-1在结肠癌组织中高度表达,提示神经内分泌参与了结肠癌的发生有关,说明P物质及NK-1受体可能参与了结肠癌的发病过程。
OBJECTIVE: To investigate the expression and localization of substance P and its receptor neurokinin 1 (NK-1) in colon cancer and to explore its significance in the pathogenesis and clinical diagnosis of colon cancer. Methods: Immunohistochemistry was used to detect the expression of SP and NK-1 in normal colon, colon cancer and adjacent tissues. Using jmtjfx10 [1] .31 statistical software processing data, the data obtained Q test. Results: (1) Positive staining of substance P in the cytoplasm of colon cancer showed nests or diffuse distribution. ②NK-1 receptor mainly in cytoplasm, nests or diffuse distribution; a few in the cell membrane. ③ substance and its receptor NK-1 expression in normal colonic mucosa and adjacent tissues, located in the cytoplasm, mostly round, oval or irregular shape. (4) The expression of p-substance and its receptor, NK-1, was highly expressed in colon cancer tissues, which was significantly higher than that in normal colorectal tissues and adjacent tissues (p <0.01) Suggesting that neuroendocrine involved in the occurrence of colon cancer, indicating substance P and NK-1 receptors may be involved in the pathogenesis of colon cancer.