细胞因子信号抑制因子在Behet病患者外周血单核细胞的表达

• 作 者: 王红 刘云霞 彭晓燕 王寅琳
• 机 构: 首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科学与视觉科学重点实验室,山东省济南市第四人民医院眼科,
• 出 处: 眼科
• 发表日期: 2010年03期
• 关键词: 外周血单核细胞 Beh 细胞因子 et SOCS 葡萄膜炎 抑制因子 cytokine 蛋白免疫印迹 实时荧光定量

目的探讨Behet病患者外周血单核细胞(peripheral blood mononuclear cell,PBMC)细胞因子信号抑制因子(sup-pressor of cytokine signaling,SOCS)表达以及SOCS在Behet病发病机制中的作用。设计病例-对照研究。研究对象16例Behet病患者及16例正常人。方法取Behet病患者和正常人的静脉血,用ELISA方法检测血清中细胞因子IL-4、IL-12、IFN-γ的水平;用实时荧光定量PCR和蛋白免疫印迹方法检测外周血单核细胞内SOCS基因和蛋白的表达。主要指标Behet病患者和健康人之间IL-4、IL-12、IFN-γ、CIS、SOCS1、SOCS2、SOCS3、SOCS5含量。结果 IL-4、IL-12和IFN-γ在Behet病患者血清中的水平均较正常对照组显著升高(P均<0.05)。相对荧光定量PCR结果显示含SH2结构的细胞因子诱导蛋白(cytokinein-ducible SH2 containing protein,CIS)、SOCS1、SOCS2、SOCS3、SOCS 5mRNA在Behet病患者PBMC内的表达分别是正常对照组的0.42、1.19、1.31、0.64和1.16倍。蛋白免疫印迹结果显示Behet病患者的CIS水平显著低于正常人(P<0.01),SOCS1明显高于正常人(P<0.01),SOCS2在患者和正常对照组之间无显著差异(P>0.05),SOCS3低于正常对照组(P<0.01),SOCS5高于正常对照组(P<0.01)。结论 Behet病患者PBMC内SOCS1和SOCS5水平升高、CIS和SOCS3水平降低与Behet病Th1型反应增强有关。SOCS5在Behet病发生过程中可能起保护作用。

Objective To investigate the expression of cytokine signaling (SOCS) in peripheral blood mononuclear cells (PBMC) and the role of SOCS in the pathogenesis of Behet disease in Behet disease. Design Case-Control Study. The study included 16 patients with Behet disease and 16 normal subjects. Methods The venous blood of patients with Behet disease and normal people were collected. The levels of IL-4, IL-12 and IFN-γ in serum were detected by ELISA. The levels of IL-4, IL-12 and IFN- Expression of SOCS gene and protein in nucleus. IL-4, IL-12, IFN-γ, CIS, SOCS1, SOCS2, SOCS3 and SOCS5 levels among patients with Behet disease and healthy subjects. Results The serum levels of IL-4, IL-12 and IFN-γ in patients with Behet disease were significantly higher than those in normal controls (all P <0.05). Relative fluorescence quantitative PCR results showed that the expression of cytokine-ducing SH2 containing protein (CIS), SOCS1, SOCS2, SOCS3 and SOCS 5 mRNA in PBMC of patients with Behet disease were 0.42 , 1.19, 1.31, 0.64 and 1.16 times. Western blot results showed that the level of CIS in Behet disease patients was significantly lower than that in normal controls (P <0.01), SOCS1 was significantly higher than that in normal controls (P <0.01), and SOCS2 was not significantly different between patients and controls > 0.05), SOCS3 was lower than that of the normal control group (P <0.01), SOCS5 was higher than that of the normal control group (P <0.01). Conclusion The levels of SOCS1 and SOCS5 in PBMC of Behet disease patients are elevated, and the decrease of CIS and SOCS3 level is associated with the increased Th1-type response in Behet’s disease. SOCS5 may play a protective role in the pathogenesis of Behet disease.