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目的对中国六省分离的伤寒沙门菌进行脉冲场凝胶电泳(PFGE)分析,初步建立中国伤寒沙门菌的数据库。方法采用PFGE技术,对中国1979-2005年六省分离的伤寒沙门菌用XbaⅠ酶切,用BioNumerics软件对PFGE图谱进行分析,并且建立中国分子分型数据库。结果收集的417株菌株共有288种不同的PFGE带型,相似性系数在55.2%～100%之间,不同的省份和年份之间有交叉带型。结论中国伤寒沙门菌的菌株变异较大,不同的省份可能存在不同克隆系的伤寒沙门菌菌株的流行。通过建立中国伤寒沙门菌PFGE带型数据库,对中国以后伤寒沙门菌的监测具有重要的意义。
Objective To analyze the PFGE of Salmonella typhi isolated from six provinces in China and to establish a database of Salmonella typhi in China. Methods The PFGE technique was used to digest Salmonella typhi isolated from six provinces in China from 1979 to 2005 with Xba Ⅰ digestion. BioNumerics software was used to analyze the PFGE patterns and to establish a Chinese molecular typing database. Results A total of 417 isolates collected 288 different PFGE patterns with similarity coefficients ranging from 55.2% to 100%. Cross-band patterns were found between different provinces and years. Conclusions The strains of Salmonella typhi in China vary greatly, and different strains of Salmonella typhi in different climates may exist in different provinces. Through the establishment of Chinese Salmonella typhi PFGE band database, the monitoring of Salmonella typhi in China is of great significance.