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目的探讨异常黑胆质证肝癌种植模型大鼠下丘脑-垂体-肾上腺轴功能紊乱状态,诠释异常黑胆质肿瘤高发的生物学基础。方法选取雄性SD大鼠36只,稳定饲养3d,随机分为对照组、单纯肝癌种植组、异常黑胆质证肝癌种植组,每组12只,对照组给予普通鼠饲料饲养3w后行假肝癌种植术(肝内注射生理盐水),单纯肝癌种植组给予普通鼠饲料饲养3w后行肝内注射walker-256瘤细胞手术,异常黑胆质证肝癌种植组采用足底电击、干寒饲料、制动等多种因素刺激3w建立异常黑胆质证载体大鼠模型,再通过肝内注射walker-256瘤细胞手术建立异常黑胆质证肝癌种植大鼠模型,3组大鼠均于术后2w后取材,光镜观察各组大鼠下丘脑、垂体、肾上腺组织形态结构变化,并采用酶联免疫方法测定各组大鼠血浆中促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ATCH)和皮质酮(CORT)的含量变化并进行统计分析。结果与对照组相比,异常黑胆质证肝癌种植组大鼠下丘脑、垂体、肾上腺组织均呈现不同改变,血浆CRH、ATCH含量明显升高,差异有统计学意义(P<0.05),;与单纯肝癌种植组相比,异常黑胆质证肝癌种植组大鼠血浆CRH含量明显升高,差异有统计学意义(P<0.05)。结论异常黑胆质证肝癌大鼠下丘脑-垂体-肾上腺轴功能处于亢进状态,其功能紊乱可能是影响异常黑胆质肿瘤发展的生物学基础。
Objective To investigate the dysfunction of the hypothalamus-pituitary-adrenal axis in rats with abnormal savda hepatocellular carcinoma and to explain the biological basis of abnormal savda tumors. Methods Thirty-six male Sprague-Dawley rats were randomly divided into three groups: control group, HCC implantation group and abnormal hepatobiliary HCC implantation group, with 12 rats in each group. The control group was fed with normal rat diet for 3 weeks, (Intraperitoneal injection of normal saline), simple liver cancer group were given normal rat feed 3w after intrahepatic injection of walker-256 tumor cell surgery, abnormal savda syndrome liver cancer implantation group using plantar shock, dry and cold feed, braking, etc. A variety of factors to stimulate the 3w to establish an animal model of abnormal Savda vector, and then through intrahepatic injection of walker-256 tumor cells to establish abnormal savda hepatica plantation rat model, 3 groups of rats were drawn 2w after surgery, light The morphological changes of hypothalamus, pituitary and adrenal gland in each group were observed by microscope. The levels of corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ATCH) and corticosterone (CORT) content changes and statistical analysis. Results Compared with the control group, the hypothalamus, pituitary and adrenal tissues of rats in abnormal savda hepatocarcinoma group showed different changes, the levels of plasma CRH and ATCH were significantly increased (P <0.05), and Compared with the HCC group, the levels of plasma CRH were significantly increased in the abnormal hepatobiliary implantation group, the difference was statistically significant (P <0.05). Conclusion Hypothalamus-pituitary-adrenal axis function in hyperthyroidism hepatocarcinoma rats is hyperthyroidism, and its dysfunction may be the biological basis for the development of abnormal savda tumors.