阿霉素、5-Fu、三尖杉酯碱及去炎松对体外培养的人视网膜色素上皮细胞的抑制

• 作 者: 王兆艳 惠延年 胡明
• 机 构: 西安第四军医大学西京医院眼科!710032,西安第四军医大学西京医院眼科!710032,西安第四军医大学西京医院眼科!710032
• 出 处: 眼科研究
• 发表日期: 1996年01期
• 关键词: Fu 阿霉素 细胞抑制率 胸腺嘧啶核苷 5-氟尿嘧啶 三尖杉酯碱 去炎松 体外培养 抑制率 抑制作用

应用3H-胸腺嘧啶核苷（3H－TdR）掺入及液体闪烁测量技术，观察不同浓度的阿霉素，5-氟尿嘧啶（5－Fu）、三尖杉酯碱及去炎松对体外培养的人视网膜色素上皮细胞（RPE）增殖的抑制作用。结果；阿霉素在2～32ng／ml时，对RPE细胞抑制率为85～77.2％，ID50为12ng／ml；5－Fu在0.2～3．2μg／ml时，对RPE细胞抑制率为14．5～81．7％，ID50为0．6μg／ml；三尖杉酯碱在2．5～40ng／ml时，抑制率为18．5～94．1％，ID50为4.5ng／ml；去炎松在200～350μg／ml时对RPE细胞抑制率为37．9～53．5％，ID50为340μg／ml。结论：阿霉素、5－Fu、三尖杉酯碱及去炎松能有效地抑制RPE细胞的增殖。

3H-Thymidine (3H-TdR) incorporation and liquid scintillation measurement were used to observe the effects of different concentrations of doxorubicin, 5-fluorouracil, harringtonine and triamcinolone on cultured in vitro Inhibition of human retinal pigment epithelium (RPE) proliferation. Results The inhibitory rate of RPE cells was 85-77.2% with ID50 of 12ng / ml when adriamycin was 2 ~ 32ng / ml and that of RPE cells was 14ng with 5 ~ Fu of 0.2 ~ 3.2μg / ml .5 ~ 81.7%, ID50 0.6μg / ml; harringtonine 2.5 ~ 40ng / ml, the inhibition rate was 18.5 ~ 94.1%, ID50 was 4.5ng / ml; To pine at 200 ~ 350μg / ml on RPE cells inhibition rate of 37.9 ~ 53.5%, ID50 of 340μg / ml. Conclusion: Adriamycin, 5-Fu, harringtonine and triamcinolone can effectively inhibit the proliferation of RPE cells.