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从精子形态学检查,了解生理或病理情况下精液中正常与畸形精子所占的比例,是判断男性生育能力的一项重要指标。现有的精子染色一般用Giemsa,Wright及H·E等方法,对精子头部染色比较满意,但尾部往往显示不够清楚,亦易褪色。我们在实践中改用铁苏木素加温染色,需时较短,而且不易褪色,对精子全貌显示十分清楚,现将方法介绍如下: 1.染液配制:Ⅰ.1%苏木素原液:纯酒精100ml,苏木精1g。Ⅱ.苏木素稀释液:苏木素原液lml,甘油1ml,蒸馏水8ml。2.方法:(1)取新鲜精液放入试管内,加入少量
From sperm morphology to understand the physiological or pathological conditions, the proportion of normal and abnormal sperm in the sperm, is to determine the male fertility is an important indicator. Existing sperm staining is generally used Giemsa, Wright and H · E and other methods, the sperm head staining more satisfied, but the tail often shows not clear enough, but also easy to fade. We use iron hematoxylin in practice to warm the stain, take a short time, but not easy to fade, the whole picture of the sperm is very clear, the method described below: 1 dye preparation: Ⅰ.1% hematoxylin stock solution: pure alcohol 100ml , Hematoxylin 1g. Ⅱ. Hematoxylin Dilution: Hematoxylin solution lml, glycerol 1ml, distilled water 8ml. 2. Methods: (1) Take fresh semen into the test tube, add a small amount