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优化隐丹参酮脂质体的最佳制备工艺条件。采用溶剂分散法制备隐丹参酮脂质体,以包封率为指标,以药脂比、膜材比、反应温度和反应时间为因素,采用L9(34)正交试验设计对制备条件进行优选,透析法测定隐丹参酮脂质体的包封率,透射电镜和激光粒度仪测定其形态和粒径。溶剂分散法制备隐丹参酮脂质体的最佳工艺为:药脂质量比1∶20,膜材质量比4∶1,反应温度30℃,反应时间6min;平均包封率为(87.31±1.45)%;粒径集中分布在(176±5.65)nm,且隐丹参酮脂质体形态和粒径均匀,重现性好。
Optimization of cryptotanshinone liposome preparation process conditions. Cryptotanshinone liposomes were prepared by solvent dispersion method. The entrapment efficiency was used as an index to optimize the preparation conditions by using L9 (34) orthogonal design with factors of drug-lipid ratio, membrane ratio, reaction temperature and reaction time. The entrapment efficiency of cryptotanshinone liposomes was determined by dialysis method. The morphology and particle size were determined by transmission electron microscopy and laser particle sizer. Solvent dispersion method for preparing cryptotanshinone liposomes was as follows: the mass ratio of drug to lipid 1:20, mass ratio of membrane to membrane 4:1, reaction temperature 30 ℃, reaction time 6min, average entrapment efficiency 87.31 ± 1.45, %. The particle size distribution was concentrated in (176 ± 5.65) nm, and the shape and size of Cryptotanshinone liposomes were uniform and reproducible.