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AIM To identify hepatitis C virus(HCV)core proteinepitopes recognized by HLA-A2 restricted cytotoxic Tlymphocyte(CTL).METHODS Utilizing the method of computer predictionfollowed by a 4h~(51) Cr-release assay confirmation.RESULTS The results showed that peripheral bloodmononuclear cells(PBMC)obtained from two HLA-A2positive donors who were infected with HCV could lysaautologous target cells labeled with peptide“ALAHGVRAL(core 150-158)”.,The rates of specific lysis of the cellsfrom the two donors were 37.5% and 15.8%,respectively.Blocking of the CTL response with anti-CD4mAb caused no significant decrease of the specific lysis.But blocking of CTL response with anti-CD8 mAb couldabolish the lysis.CONCLUSION The paptide(core 150-158)is thecandidate epitope recognized by HLA-A2 restricted CTL.
AIM To identify hepatitis C virus (HCV) core proteine peptides identified by HLA-A2 restricted cytotoxic Tlymphocyte (CTL). METHODS Utilizing the method of computer prediction by circulating 4 h 51 Cr-release assay confirmation. RESULTS The results showed that peripheral blood mononuclear cells (PBMC) obtained from two HLA-A2 positive donors who were infected with HCV could lysaautologous target cells labeled with peptide “ALAHGVRAL (core 150-158)”, The rates of specific lysis of the cells from the two donors were 37.5% and 15.8%, respectively. Blocking of the CTL response with anti-CD4 mAb caused no significant decrease of the specific lysis. But blocking of CTL response with anti-CD8 mAb could abolish the lysis. CONCLUSION The paptide (core 150-158) is thecandidate epitope recognized by HLA-A2 restricted CTL.