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目的:观察外加稳恒直流电场对兔球囊损伤后腹主动脉舒张功能的影响。方法:健康日本大耳白兔40只,随机分为4组,建立兔腹主动脉球囊损伤模型,在腹主动脉两侧腰大肌埋置刺激电极。电场组术后给予稳恒直流电场刺激(电场强度4V/cm,30min/d),对照组术后不给予电场刺激,电场加N硝基-L-精氨酸甲酯(L-NAME)组和电场加L-精氨酸(L-Arg)组在术后电场刺激基础上分别经腹腔注射给予L-NAME及经胃管给予L-Arg治疗,术后1、2、4周检测血管舒张功能,硝酸还原酶法检测血管组织匀浆一氧化氮(NO)。结果:术后4周电场组对乙酰胆碱的最大舒张反应强度及NO水平[(64.65±0.53)%、(652.18±2.66)μmol/L]均显著高于对照组[(51.97±0.23)%、(637.88±5.68)μmol/L]及电场加L-NAME组[(49.57±0.82)%、(628.29±4.07)μmol/L],差异有统计学意义P<0.05,但显著低于电场加L-Arg组[(71.29±0.82)%、(657.14±3.88)μmol/L],差异有统计学意义(P<0.05)。结论:兔腹主动脉球囊损伤后外加稳恒直流电场干预4周后血管内皮舒张功能较对照组显著改善,提示外加电场有利于血管内皮功能的恢复。上述作用可能是通过NOS-NO信号通路改善血管内皮舒张功能的结果。
Objective: To observe the effect of applied steady direct current (DC) electric field on the diastolic function of abdominal aorta after balloon injury in rabbits. Methods: Forty healthy Japanese white rabbits were randomly divided into 4 groups. Rabbit abdominal aorta balloon injury models were established. Stimulation electrodes were embedded in the psoas muscle at both sides of abdominal aorta. The electric field was stimulated by steady-state DC electric field (electric field intensity 4V / cm, 30min / d), while the control group was not given electric stimulation. The electric field plus N-nitro-L-arginine methyl ester L-arginine group and L-Arg group were given intraperitoneal injection of L-Arg and L-Arg via gastric tube after electric field stimulation. The vasodilation was detected at 1, 2 and 4 weeks after operation Function, nitric acid reductase method to detect nitric oxide (NO) in vascular homogenate. Results: The intensity of maximal diastolic response to acetylcholine and the level of NO in electric field group at 4 weeks after operation were significantly higher than those in control group [(64.65 ± 0.53)% vs (652.18 ± 2.66) μmol / L [(51.97 ± 0.23)% vs (49.57 ± 0.82)%, (628.29 ± 4.07) μmol / L] in the electric field plus L-NAME group, the difference was statistically significant P <0.05, but significantly lower than the electric field plus L- Arg group [(71.29 ± 0.82)%, (657.14 ± 3.88) μmol / L], the difference was statistically significant (P <0.05). CONCLUSION: The vasodilatory function of vascular endothelial cells significantly improved after the abdominal aorta balloon injury in rabbits compared with the control group after the intervention of a steady DC field for 4 weeks, suggesting that the applied electric field is beneficial to the recovery of vascular endothelial function. The above-mentioned effect may be the result of improving the vascular endothelial relaxation function through the NOS-NO signaling pathway.