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Objective To explore the effect of p27gene on th e growth inhibition of laryngeal car cinoma cell line Hep-2.Methods The p27cDNAwas transfected into human lary ngeal carcinoma cell line Hep-2cells with lipofectamine.The cell cycle s were observed by means of FCM assay.p27expression was detected b y dot-blot hybridization and Western blot.Results Expression of p27in Hep-2was identified by Dot blot and Western blot analyses.the growt h rate of Hep-2transfected with p27g ene was markedly suppressed.Cell cy cle analysis by flow cytometry show that the number of cells in G 0 ～G 1 phase of Hep-2cells was significant ly increased while cells in S and G 2 +M phase was decreased compared with that of the control Hep-2cells.Conclusion Transduction of p27gene into lower e xpression cancer cells can restore i ts suppressive effect on cell growth by arrest of cell cycle at G 1 phase.
Objective To explore the effect of p27gene on th e growth inhibition of laryngeal car cinoma cell line Hep-2. Methods The p27 cDNA was transfected into human lary ngeal carcinoma cell line Hep-2 cells with lipofectamine. The cell cycle s were observed by means of FCM assay .p27expression was detected by dot-blot hybridization and Western blot.Results Expression of p27in Hep-2was identified by Dot blot and Western blot analyzes. The growth rate of Hep-2transfected with p27g ene was markedly suppressed. Cell cy cle analysis by flow cytometry show that the number of cells in G 0 ~ G 1 phase of Hep-2 cells was significant ly increased while cells in S and G 2 + M phase was decreased compared with that of the control Hep-2 cells. Conlusion Transduction of p27 gene into lower e xpression cancer cells can restore i ts suppressive effect on cell growth by arrest of cell cycle at G 1 phase.