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Objective:To evaluate the efficacy of Atalantia monophylla(A.monophylla)leaf in different solvent crude extracts and fractions against eggs of Spodoptera litura(S.litura).Methods:Hexane,ethyl acetate and chloroform solvent extracts of A.monophylla leaf and 12 fractions from hexane extract were screened at 5.0%t,2.5%,1.0%and 0.5%for crude extracts and 1000,500,250 and 125 mg/kg for fractions against the eggs of S.litura for the ovicidal activity.LC_(50)and LC_(90)were calculated using probit analysis.Results:Hexane crude extract showed maximum ovicidal activity of 61.94%at 5.0%concentration with a correlation value of r~2=0.81,and least LC_(50)value of3.06%.Hexane extract was fractionated using silica gel column chromatography and 12 fractions were obtained.Fraction 9 was active which showed maximum ovicidal activity of 75.61%at 1000mg/kg with the LC_(50)value of 318.65 mg/kg and LC_(90)value of 1473.31 mg/kg.In linear regression analysis,significant and high correlation(r~2=0.81%)was seen between concentration and ovicidal activity of hexane crude extracts and its active frsctien.Conclusions:As per our knowledge,this is the first report for ovicidal activity of A.monophylla against S.litura,A.monophylla could be used for the management of S.litura and other insect pests.
Objective: To evaluate the efficacy of Atalantia monophylla (A. monophylla) leaf in different solvent crude extracts and fractions against eggs of Spodoptera litura (S. litura). Methods: Hexane, ethyl acetate and chloroform solvent extracts of A. monophylla leaf and 12 fractions from hexane extract were screened at 5.0% t, 2.5%, 1.0% and 0.5% for crude extracts and 1000, 500, 250 and 125 mg / kg for fractions against the eggs of S. lora for the ovicidal activity. LC_ (50) and LC_ (90) were calculated using probit analysis. Results: Hexane crude extract showed maximum ovicidal activity of 61.94% at 5.0% concentration with a correlation value of r ~ 2 = 0.81 and least LC_ (50) value of 3.06%. Hexane extract was fractionated using silica gel column chromatography and 12 fractions were obtained. Fraction 9 was active which showed maximum ovicidal activity of 75.61% at 1000 mg / kg with the LC_ (50) value of 318.65 mg / kg and LC_ (90) value of 1473.31 mg / kg.In linear regression analysis, significant and high correlation (r ~ 2 = 0.81%) was s een between concentration and ovicidal activity of hexane crude extracts and its active frsctien.Conclusions: As per our knowledge, this is the first report for ovicidal activity of A.monophylla against S.litura, A.monophylla could be used for the management of S .litura and other insect pests.