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我们用油菜(Brassia napus L.)勺叶青的苔段为材料,进行不同照射量率和照射量的γ-射线处理,以研究苔段成苗和形态变异的影响。结果证明,相同照射量率,不同照射量处理,随着照射量的增加,油菜苔段分化成苗能力下降,表现苗分化数减少。在照射量达10860伦时,苔段已经丧失分化能力,说明10000 伦以上的照射剂量,为油菜苔段分化的致死剂量。相同照射量率,用低照射量照射,有促进苔段分化成苗的作用,表现分化数增加。而用较高照射量处理,有抑制苗分化的作用。这种抑制苗分化的作用,又随着照射量率的降低而减弱。分化的苗,我们用正常苗,异常苗和线状苗分别表示苗的形态变异。用过γ-射线处理,油菜苔段分化的苗,正常苗比例严重下降,异常苗的比例则明显增加。异常苗的比例最高可达78.2％。有趣的是线状苗的分化,在因γ-射线处理促进苗分化,或用高照射量,低照射量率处理,抑制苗分化作用减弱的两种情况下,都表现分化数增加。鉴于油菜苔段培养分化成苗,进而长成完整植株。因此通过本研究,对利用油菜苔段进行γ-射线处理,提高诱变效率,进行诱变育种,有一定指导意义。
In this study, γ-ray treatments of Brassica napus L. were carried out to study the effects of seedling development and morphological variation. The results showed that the same irradiation rate, different irradiation dose, with the increase of irradiation, rape moss differentiation into seedling ability decreased, the performance of seedlings decreased. In the dose of 10860 Lun, the moss segment has lost the ability to differentiate, indicating that more than 10,000 lun irradiation dose, the lethal dose of rape moss differentiation. The same dose rate, irradiation with a low dose, to promote the differentiation of moss into seedlings, the performance of the increased number of differentiation. With a higher exposure to deal with the role of inhibition of seedling differentiation. This inhibition of the role of seedling differentiation, but also with the dose rate decreased and weakened. Differentiated shoots, we use normal shoots, abnormal shoots and linear shoots, respectively, represent the morphological changes of shoots. Used γ-ray treatment, rape moss differentiation of seedlings, a serious decline in the proportion of normal seedlings, the proportion of abnormal seedlings increased significantly. The proportion of abnormal seedlings up to 78.2%. Interestingly, the differentiation of linear shoots showed an increase in the number of differentiations in both cases where shoot differentiation was promoted by γ-ray treatment, treatment with a high irradiation dose or a low irradiation dose, and inhibition of the differentiation of shoots. In view of rape moss differentiation into seedlings, and then grow into a complete plant. Therefore, through this study, the use of rape moss y-ray treatment to improve the mutagenesis efficiency, mutagenesis breeding, a certain guiding significance.