Identification of Intergeneric Hybrid Plants Between Oryza sativa and O. minuta via GISH and RAPD

• 作 者: YU Shun-wu CHEN Bao-tang TAO A
• 机 构: National Key Laboratory of Crop Genetic Improvement,Plant Biotechnology Research Center
• 出 处: 中国农业科学
• 发表日期: 2004年期
• 关键词: Genomic in situ hybridization Hybrid Oryza minuta Oryza sativa Blast
• 基金项目: 国家高技术研究发展计划(863计划)

To transfer desirable resistance traits from O. minuta to O. sativa, intergeneric hybrid plants between O. sativa (AA, 2n=2X=24) and O. minuta (BBCC, 2n=4X=48) were produced by embryo rescue after sexual cross. Morphological observation and chromosome counts indicated their hybrid status (ABC, 2n =3X=36). Genomic in situ hybridization (GISH) was further applied to confirm the parentage of the chromosomes of F1 hybrids. Chromosomes of O. minuta and O. sativa were distinguishable in the hybrids in different fluorescence colors. GISH indicated that A and BC chromosomes were not randomly assembled in a cell.RAPD profiles unequivocally revealed their hybrids with double parent patterns. The results of blast tests showed that the hybrids had obtained disease resistance from O. minuta, and had a level of susceptibility between the parents.