内毒素诱导急性肺损伤小鼠支气管肺泡灌洗液中谷氨酸含量变化的初步研究

• 作 者: 罗志峰 王乐 李晨 岳少杰 汉建忠 许建平 刘惠君 黄艳红 廖晓红 罗自强
• 机 构: 中南大学湘雅医学院,湘南学院基础医学课部,中南大学湘雅医院,
• 出 处: 湘南学院学报(医学版)
• 发表日期: 2010年01期
• 关键词: 内毒素 急性肺损伤 肺泡灌洗液 谷氨酸

目的观察LPS诱导急性肺损伤时肺内是否存在谷氨酸(Glu)的释放。方法腹腔注射内毒素制备小鼠急性肺损伤模型,于6h后采用生理盐水行支气管肺泡灌洗,用高效液相色谱法测量灌洗液中氨基酸的浓度。结果与生理盐水对照组相比,LPS处理组支气管肺泡灌洗液中Glu的浓度增高(P<0.01);苏氨酸、蛋氨酸及精氨酸的浓度降低(P<0.05),其它氨基酸浓度无显著性变化。结论在LPS诱导的肺损伤过程中肺组织中部分氨基酸代谢发生显著变化,肺内谷氨酸的释放增多,进一步提示Glu参与了内毒素诱导的肺损伤的病理生理过程。

Objective To observe the presence or absence of glutamate (Glu) release in the lungs of LPS-induced acute lung injury. Methods The model of acute lung injury induced by intraperitoneal injection of endotoxin was established. After 6 hours, bronchoalveolar lavage was performed with saline and the concentration of amino acids in lavage fluid was measured by high performance liquid chromatography. Results Compared with saline control group, the concentration of Glu in bronchoalveolar lavage fluid of LPS treatment group was increased (P <0.01), and the concentrations of threonine, methionine and arginine were decreased (P <0.05). The concentrations of other amino acids Significant changes. CONCLUSIONS: Some amino acid metabolism in lung tissue changes significantly during LPS-induced lung injury and the release of glutamate in the lung is increased, suggesting that Glu is involved in the pathophysiological process of endotoxin-induced lung injury.