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白细胞介素2(IL-2)是T淋巴细胞产生的免疫调节因子,是Ts、Tc细胞分化增殖必需的调节因子。近年来研究表明,IL-2与临床很疾病有关,如肿瘤、高血压以及自射免疫性疾病等。本文报告急性心肌梗塞及甲克患者IL-2水平的变化。 IL-2测定方法: 对照组及实验组肝素抗凝取血分离外周血单个核细胞(PBMC),置于含完全1640培养液瓶中,细胞浓度为1×10~6个/ml,加入PHA使终浓度为100μg/ml,37℃、5％CO_2培养24小时,离心弃上清,以不含PHA的全1640继续培养44小时,离心收集上清。再将标准IL-2行倍比稀释,以克隆化的IL-2依赖株(CTLL)~3H-TdR参入率为指标反映IL-2的活性。待测上清中的IL-2活性单位即可用半对数作图法确定。
Interleukin-2 (IL-2) is an immunomodulatory factor produced by T lymphocytes and is a necessary regulatory factor for the proliferation of Ts and Tc cells. In recent years, studies have shown that, IL-2 and clinical diseases, such as cancer, hypertension and autoimmune diseases. This article reports the changes of IL-2 levels in patients with acute myocardial infarction and a gram. IL-2 assay: Control group and experimental group heparin anticoagulant blood was collected from peripheral blood mononuclear cells (PBMC), placed in a complete 1640 medium containing the concentration of 1 × 10 6 cells / ml, adding PHA The final concentration of 100μg / ml, 37 ℃, 5% CO_2 cultured for 24 hours, the supernatant was centrifuged to PHA-free 1640 to continue training for 44 hours, the supernatant was collected by centrifugation. Then, the IL-2 was diluted by the standard IL-2, and the IL-2 activity was measured by the incorporation rate of the cloned IL-2-dependent strain (CTLL) ~ 3H-TdR. IL-2 activity units in the supernatant can be determined by semi-logarithmic mapping.