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以人参锈腐病病株根部分离的菌株R2为试材,采用传统的形态学观察与现代分子生物学技术相结合的方法进行了病原菌鉴定,并研究其生物学特性。结果表明:该病原菌为粪壳菌纲(Sordariomycetes),肉座菌目(Hypocreales),叶赤壳科(Nectriaceae),柱孢属(Cylindrocarpon),毁灭柱孢菌(Cylindrocarpon destructans)。该菌在马铃薯蔗糖琼脂培养基(PDA)和察氏培养基(CZA)上生长较快,最适的碳、氮源为蔗糖和尿素,最适生长温度为20~25℃,最适pH值为中性偏酸环境。
The strain R2 isolated from the roots of ginseng root rot was used as material to identify the pathogen and to study the biological characteristics of the strain R2 by combining the traditional morphological observation with modern molecular biology techniques. The results showed that the pathogen was Sordariomycetes, Hypocreales, Nectriaceae, Cylindrocarpon and Cylindrocarpon destructans. The strain grew faster on potato sucrose agar medium (CZA) and Czochralski (CZA). The most suitable carbon and nitrogen sources were sucrose and urea. The optimum growth temperature was 20-25 ℃, and the optimum pH Neutral acidic environment.