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目的:建立以高效液相色谱法测定健脾颗粒中橙皮苷含量的方法。方法:色谱柱为Kromasil C18(200mm×4.6mm,5μm),流动相为乙腈-0.3%磷酸溶液(20∶80),检测波长为283nm,流速为1.0mL·min-1。结果:橙皮苷的检测浓度在9.92～99.75μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为99.1%,RSD=0.2%(n=6)。结论:本方法简便、准确、重现性好,可用于健脾颗粒的质量控制。
Objective: To establish a method for determination of hesperidin in Jianpi Granules by HPLC. Methods: The chromatographic column was Kromasil C18 (200mm×4.6mm, 5μm). The mobile phase was acetonitrile-0.3% phosphoric acid solution (20∶80). The detection wavelength was 283nm and the flow rate was 1.0mL·min-1. Results: The detection concentration of hesperidin in the range of 9.92～99.75μg·mL-1 showed a good linear relationship with the integrated value of the peak area (r=0.9999); the average recovery was 99.1%, and the RSD was 0.2% (n=6). . Conclusion: This method is simple, accurate and reproducible, and can be used for the quality control of Jianpi Granules.