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目的:从灵芝提取物中分离纯化灵芝酮二醇,并探讨其对8种肿瘤细胞的抑制作用。方法:灵芝氯仿提取部分经反复硅胶柱层析得到一个白色针晶,经熔点测定1、H-NMR和13C-NMR数据分析以及与已知化合物比较,确定其化学结构。利用MTT法检测其对人Burkitt淋巴瘤细胞株CA46、人胃癌细胞株SGC7901、人结肠癌细胞株SW1116、人结肠癌细胞株SW480、人肝癌细胞株SMMC7221、人肝癌细胞株HepG2、人髓系白血病细胞株HL60、人慢性粒细胞白血病细胞株K562的生长抑制情况。结果:经理化性质鉴定、波谱分析,确定白色针晶结构为24(S),25-二羟基-5α-羊毛甾-7,9(11)-二烯-3-酮,即灵芝酮二醇。MTT结果表明,灵芝酮二醇对HL60和K562 2种瘤株有较强抑制作用,对CA46、SMMC7221、HepG2、SW480、SW1116及SH-SY5Y6种瘤株抑制作用较弱。结论:灵芝三萜类成分灵芝酮二醇对人白血病肿瘤细胞HL60及K562的增殖有一定的抑制作用,初步确定灵芝酮二醇为灵芝抗白血病的活性成分之一。
OBJECTIVE: To isolate and purify Ganoderma lucidum ketone diol from Ganoderma lucidum extract and investigate its inhibitory effect on 8 kinds of tumor cells. Methods: A white needle crystal was obtained by repeated silica gel column chromatography. The melting point was determined by 1H-NMR and 13C-NMR data and compared with the known compounds to confirm the chemical structure. Human Burkitt’s lymphoma cell line CA46, human gastric cancer cell line SGC7901, human colon cancer cell line SW1116, human colon cancer cell line SW480, human hepatoma cell line SMMC7221, human hepatoma cell line HepG2, human myeloid leukemia Cell line HL60, human chronic myelogenous leukemia cell line K562 growth inhibition. Results: The physiochemical characterization and spectral analysis showed that the white needle crystal structure was 24 (S), 25-dihydroxy-5α-lanosta-7,9 (11) -dien- . The results of MTT showed that Ganoderma lucidum ketone diol had a strong inhibitory effect on HL60 and K562 strains, and had weaker inhibitory effects on CA46, SMMC7221, HepG2, SW480, SW1116 and SH-SY5Y6. CONCLUSION: Ganoderma lucidum, a triterpene glycoside, inhibits the proliferation of human leukemia cell lines HL60 and K562, and is one of the active ingredients of Ganoderma lucidum against leukemia.