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AIM: To investigate the expression of Fos and Jun proteins in rat brains after focal cerebral ischemia followed by reperfusion and effects of RSM and astragus.METHODS: 30 SD adult male rats were divided into 5 groups at random .Group A:sham operated group;Group B:model group;Group C:treated with RSM;Group D:treated with astragus;Group E:treated with RSM and astragus.The immunohistochemistry and medical image processing system(MIPS)were used to measure the numbers and mean grey levels of Fos and Jun protein positive cells in rat cerebral cortex of 5 groups. RESULTS: (1) In cerebral cortex of group B ,C ,D ,E,the numbers of Fos and Jun positive cells were more than those in group A and mean grey levels of Fos and Jun positive cells were lower than those in group A(P< 0.01);(2) In cerebral cortex of ischemic sides in group C,D,E,the numbers of Fos and Jun positive cells were less than those in group B and mean grey levels of Fos and Jun positive cells were higher than those in group B(P< 0.01) ;(3) Group E had more significant effects than group C or group D (P< 0.01). CONCLUSION: The expression of Fos protein and Jun protein in model group increased significantly,compared with sham operated group;RSM ,astragus ,RSM and astragus all could inhibit partly the expression of Fos protein and Jun protein after cerebral ischemia and reperfusion;Prescription of RSM and astragus had stronger inhibiting effects than RSM or astragus.It may be one of mechanisms that ischemic stoke is treated by reinforcing Qi and activating blood circulation therapy in TCM clinic.
AIM: To investigate the expression of Fos and Jun proteins in rat brains after focal cerebral ischemia followed by reperfusion and effects of RSM and astragus. METHODS: 30 SD adult male rats were divided into 5 groups at random. Group A: sham operated group; Group B: model group; Group C: treated with RSM; Group D: treated with astragus; Group E: treated with RSM and astragus. Immunohistochemistry and medical image processing system (MIPS) were used to measure the numbers and mean gray levels of Fos and Jun protein positive cells in rat cerebral cortex of 5 groups. RESULTS: (1) In cerebral cortex of group B, C, D, E, the numbers of Fos and Jun positive cells were more than those in group A and mean gray levels of Fos and Jun positive cells were lower than those in group A (P <0.01); (2) In cerebral cortex of ischemic sides in group C, D, E, the numbers of Fos and Jun positive cells were less than those in group B and mean gray levels of Fos and Jun positive cells were higher than those in group B (P < 0.01); (3) Group E had more significant effects than group C or group D (P <0.01). CONCLUSION: The expression of Fos protein and Jun protein in model group increased significantly, compared with sham operated group; RSM, astragus, RSM and astragus all could result partly in the expression of Fos protein and Jun protein after cerebral ischemia and reperfusion; Prescription of RSM and astragus had stronger inhibiting effects than RSM or astragus. It may be one of the mechanisms that ischemic stoke is treated by reinforcing Qi and activating blood circulation therapy in TCM clinic.