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巨噬细胞泡沫样改变是动脉粥样硬化(AS)的特征性变化,胆固醇外流受阻是引起巨噬细胞泡沫样改变的重要环节之一。该文拟采用氧化低密度脂蛋白(ox-LDL)诱导巨噬细胞源性泡沫细胞的形成,然后用不同浓度的阿魏酸对巨噬细胞源性泡沫细胞进行处理,观察阿魏酸对ox-LDL诱导巨噬细胞泡沫样化过程中细胞内脂质代谢、胆固醇流出及介导胆固醇流出的ATP结合盒转运蛋白A1(ABCA1)和ATP结合盒转运蛋白G1(ABCG1)的mRNA和蛋白水平的影响,探讨阿魏酸抗AS的可能机制。结果显示,与对照组相比,ox-LDL处理组细胞内脂质含量明显增加,主要表现为胆固醇酯的含量增加;用阿魏酸处理泡沫样细胞后,细胞内脂质聚集明显减少,尤其是胆固醇酯的含量明显降低,同时胆固醇的流出也显著增加;研究还发现用不同浓度阿魏酸处理泡沫样细胞后,细胞表面ABCA1和ABCG1的mRNA和蛋白表达水平均升高。综上所述,阿魏酸可能通过增加巨噬细胞源性泡沫细胞表面ABCA1和ABCG1的表达水平,促进胆固醇流出,从而发挥抗AS的作用。
Macrophage-like foam-like changes are characteristic features of atherosclerosis (AS), and obstruction of cholesterol efflux is one of the important links that causes foam-like changes in macrophages. This article intends to use oxidized low-density lipoprotein (ox-LDL) to induce the formation of macrophage-derived foam cells, and then treated with different concentrations of ferulic acid on macrophage-derived foam cells to observe the effect of ferulic acid on ox -LDL induces intracellular lipid metabolism, cholesterol efflux and mRNA and protein levels of ATP-binding cassette transporter A1 (ABCA1) and ATP binding cassette transporter G1 (ABCG1) during macrophage foam-like processes Influence, explore the possible mechanism of ferulic acid against AS. The results showed that, compared with the control group, ox-LDL treatment group intracellular lipid content increased significantly, mainly as cholesterol ester content; with ferulic acid treatment of foam-like cells, significantly reduced intracellular lipid accumulation, especially The content of cholesterol esters decreased significantly and the cholesterol efflux also increased significantly. The results also showed that the mRNA and protein expression of ABCA1 and ABCG1 on the cell surface were increased after treated with different concentrations of ferulic acid. In summary, ferulic acid may play an anti-AS role by increasing the expression of ABCA1 and ABCG1 on the surface of macrophage-derived foam cells and promoting cholesterol efflux.