论文部分内容阅读
目的:探讨牛珀至宝丹对老年记忆减退大鼠学习记忆能力及脑TGF-β1表达和NPCs增殖的影响。方法:以成年大鼠平均逃避潜伏期的均值加两倍标准差和加一倍标准差为参照标准,将老年大鼠随机分为记忆正常组、记忆减退组、牛珀小剂量组及牛珀大剂量组。牛珀至宝丹0.05g/kg、0.1g/kg连续给药15d后,BrdU标记处于增殖状态的NPCs,Morris水迷宫进行学习记忆能力检测,免疫组化检测鼠脑尾壳核、皮质区TGF-β1表达及SVZ、DG部位BrdU阳性细胞数。结果:定位航行实验第4～5d,牛珀大剂量组大鼠平均潜伏期明显缩短,与记忆减退组比较,有显著性差异(P<0.05);空间探索实验,牛珀大剂量组大鼠第Ⅱ象限停留时间、第一次平台穿越时间、穿越平台次数与记忆减退组比较,有显著性差异(P<0.05);牛珀大剂量组大鼠脑尾壳核及皮质区TGF-β1蛋白表达明显增多,SVZ及DG部位BrdU阳性细胞数明显增加,与记忆减退组比较,有显著性差异(P<0.05)。结论:牛珀至宝丹对老年记忆减退大鼠学习记忆能力有明显促进作用,能显著促进内源性NPCs的增殖,其机理可能与牛珀至宝丹增强TGF-β1蛋白在大鼠脑内的表达有关。
Objective: To investigate the effects of Niupo Zhibao on learning and memory ability, brain TGF-β1 expression and NPCs proliferation in aged memory deficient rats. Methods: Aged rats were randomly divided into normal memory group, memory loss group, Niupo low-dose group and Niupo-Dazao group according to the average escape latency mean plus two times of standard deviation plus one standard deviation of adult rats as reference standard Dose group. NiuPo to Po Dan 0.05g / kg, 0.1g / kg for 15d after continuous administration, BrdU labeled proliferation in NPCs, Morris water maze learning and memory ability detection, immunohistochemistry detection of rat brain putamen nucleus, cortex TGF- β1 expression and the number of BrdU positive cells in SVZ and DG. Results: The average latency of rats in high-dose Niu-Piao group was significantly shorter than that in the memory-deficient group (P <0.05) at 4 ~ 5 days after positioning navigation experiment. In space exploration experiment, (P <0.05). The expression of TGF-β1 in the caudate putamen and cortex in the high-dose group of Niupo was significantly higher than that in the memory-deficient group (P <0.05) The numbers of BrdU positive cells in SVZ and DG were significantly increased, which were significantly different from those in memory deficit group (P <0.05). Conclusion: Niupo ZhiBao Dan can significantly promote the learning and memory ability of aged memory deficient rats, and can significantly promote the proliferation of endogenous NPCs. The mechanism may be related to the increased expression of TGF-β1 protein in rat brain related.