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近年来金属络合物的高效液体色谱研究发展甚为迅速,其原因是许多金属离子与有机配位体形成的络合物在紫外或可见区都有特征吸收峰。通过柱前或柱后衍生作用可以利用紫外一可见分光光度检测器检测金属离子。并且,柱前衍生与萃取相结合可以同时完成试样的衍生、净化和富集以提高分析的灵敏度。吡啶偶氮类是一类灵敏的显色剂,已在分光光度法中得到广泛的使用。但对这类显色剂与金属离子所形成络合物的高效液体色谱研究
In recent years, high performance liquid chromatography of metal complexes has developed rapidly because of the characteristic absorption peaks of many complexes of metal ions with organic ligands in the UV or visible region. By pre-column or column derivatization UV-visible spectrophotometric detection of metal ions can be used. Moreover, the combination of pre-column derivatization and extraction can simultaneously derive, purify and enrich the sample to improve the sensitivity of the analysis. Pyridine azo is a type of sensitive developer that has been widely used in spectrophotometry. However, the high performance liquid chromatographic studies of the complexes formed by such color developers with metal ions