论文部分内容阅读
目的:评估上海地区汉族脑梗死患者对氧磷酶1(PON1)对应基因的-162A/G多态性与脑梗死(CI)间的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对126例CI患者(CI组)及131名同期体检者(对照组)的PON1基因-162A/G多态性进行分析。结果:PON1基因-162A/G多态性AA型、AG型及GG型基因型在对照组和CI组中分别占22.14%、19.08%、58.78%和23.81%、37.30%、38.89%。A、G等位基因频率分布在对照组和CI组中分别为31.68%、68.32%和42.46%、57.54%。经检验,PON1基因-162A/G位点基因型和等位基因频率在2组人群中的分布差异均有统计学意义(P均<0.05),CI组的等位基因A与对照组比较,其比数比为1.5914,95%可信区间为1.1095～2.2828。结论:PON1基因-162 A/G多态性可能与CI发病有关。
Objective: To evaluate the relationship between -162A / G polymorphism of paraoxonase 1 (PON1) gene and cerebral infarction (CI) in Han patients with cerebral infarction in Shanghai. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of PON1 gene -162A / G in 126 patients with CI (CI group) and 131 controls (control group) Sex analysis. Results: The genotypes AA, AG and GG of -162A / G polymorphism of PON1 gene accounted for 22.14%, 19.08%, 58.78% and 23.81%, 37.30% and 38.89% of the control and CI groups, respectively. The frequencies of A and G alleles were 31.68%, 68.32% and 42.46%, 57.54% in the control and CI groups, respectively. The distribution of genotype and allele frequency of -162A / G in PON1 gene was significantly different between two groups (all P <0.05). Compared with control group, The ratio of the ratio of 1.5914, 95% confidence interval of 1.1095 ~ 2.2828. Conclusion: PON1 gene -162 A / G polymorphism may be related to the pathogenesis of CI.