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目的建立八角茴香水溶性成分的HPLC指纹图谱方法及莽草酸的快速定量分析方法。方法采用Waters XBirdge C_(18)色谱柱(250mm×4.6mm,5μm);流动相为0.05%磷酸水溶液(A相)-乙腈(B相),梯度洗脱;柱温25℃;流速0.5ml/min;检测波长210nm、254nm。结果对18批八角茴香药材和2批莽草及不同部位进行测定,建立了八角茴香水溶性成分HPIC指纹图谱方法和莽草酸快速定量分析方法,并对不同来源、等级和部位的八角茴香中莽草酸含量进行了分析。此外,将所建立的方法用于八角茴香伪品莽草的分析研究。结论此方法简便、可靠,为建立八角茴香的质量标准提供了依据。
OBJECTIVE To establish an HPLC fingerprinting method for the water-soluble fraction of star anise and a rapid quantitative analysis method of shikimic acid. The mobile phase consisted of 0.05% aqueous phosphoric acid (A phase) - acetonitrile (phase B) with gradient elution. The column temperature was 25 ℃ and the flow rate was 0.5 ml / min; detection wavelength 210nm, 254nm. Results 18 batches of star anise medicinal materials and 2 batches of Mangcao and different parts were determined. The HPIC fingerprinting method and rapid quantitative analysis method of water-soluble fraction of star anise were established. The effects of different sources, Oxalic acid content was analyzed. In addition, the established method was applied to the analysis of the star anise fake Mangcao. Conclusion This method is simple and reliable, which provides a basis for establishing the quality standard of star anise.